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- 2015
- Xanthomonas oryzae
- multispectral and multimodal microscopy
- spectroscopic imaging
- plant cell diseases
- classification
- rice
- Applied Physics
- University of Sciences, Technique and Technology Bamako
- Institut National Polytechnique Felix Houphou?t-Boigny Yamoussoukro
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Identification of Bacterial Diseases in Rice Plants Leaves by the Use of Spectroscopic Imaging
摘要： Rice is staple in the African habitats menu. Bacterial wilt (BLB) and leaf streak (BLS) are some of the phytopathological diseases which restrain rice production around the world. In this paper, multi-spectral and multi- modal imaging techniques have been developed to characterize the rice leaves with symptoms of bacterial wilt (BLB) and leaf streak (BLS), and to provide information on their effects, in order to reduce their spread. First, we recorded microscopic and spectroscopic images of the samples using multimodal and multispectral microscope, with spectral region ranging from UV to NIR, for each mode. Then, we extracted the spectral footprints of the cells constituents, in transmission, reflection and scattering from the spectral images. Applying multivariate statistical analysis methods to this optical spectra allowed us to characterize the effect of bacterial rice leaves caused by Xanthomonas oryzae strains. The results of the proposed technique can be useful for easy identification of this type of infection, and can serve as routine approach in biochemical and agronomic laboratories.
关键词： Xanthomonas oryzae,multispectral and multimodal microscopy,spectroscopic imaging,plant cell diseases,classification,rice
更新于2025-09-09 09:28:46
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Visible-light optical coherence tomography-based multimodal system for quantitative fundus autofluorescence imaging
摘要： Fundus autofluorescence (FAF) imaging is commonly used in ophthalmic clinics for diagnosis and monitoring of retinal diseases. Lipofuscin in the retinal pigment epithelium (RPE), with A2E as its most abundant component and a visual cycle by-product, is the major fluorophore of FAF. Lipofuscin accumulates with age and is implicated in degenerative retinal diseases. The amount of lipofuscin in RPE can be assessed by quantitative measurement of FAF. However, the currently available FAF imaging technologies are not capable of quantifying the absolute intensity of FAF, which is essential for comparing images from different individuals, and from the same individual over time. One major technical difficulty is to compensate the signal attenuation by ocular media anterior to the RPE (pre-RPE media). FAF intensity is also influenced by fluctuations in imaging conditions such as illumination power and detector sensitivity, all of which need to be compensated. In this review, we present the concept and research progress of using visible-light optical coherence tomography-based simultaneous multimodal retinal imaging to compensate signal attenuation by pre-RPE media and the influence of parameters of the acquisition system for accurate measurement of FAF intensities.
关键词： fundus autofluorescence imaging,multimodal imaging,retinal pigment epithelium lipofuscin,Visible-light optical coherence tomography,retinal imaging,fluorescence quantification
更新于2025-09-09 09:28:46
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[IEEE 2018 International Conference on Advances in Computing, Communications and Informatics (ICACCI) - Bangalore, India (2018.9.19-2018.9.22)] 2018 International Conference on Advances in Computing, Communications and Informatics (ICACCI) - Feature level fusion of Face and Iris using Deep Features based on Convolutional Neural Networks
摘要： In this work, we have proposed novel deep CNN framework architectures that effectively represent complex image characteristics which performs feature extraction in just two convolution layers and has successfully proved to be an reliable biometric verification system on employment of physiological face and iris for our system development. Extensive traits experiments in configuring the CNN hyper parameters such as number of convolution layers required, filters and its size in each layer, batch size, epochs, iterations and learning rate is a paramount, determining these factors truly depends on the nature of data and its size. Our work has relinquished our novel idea and has obtained 99% of GAR in unimodal biometric verification system itself and definitely the approach has rendered great results when compared with conventional feature extraction and classification techniques.
关键词： Verification,Multimodal biometric,CNN,Fusion
更新于2025-09-09 09:28:46
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SkImager: a concept device for <i>in-vivo</i> skin assessment by multimodal imaging
摘要： A compact prototype device for diagnostic imaging of skin has been developed and tested. Polarized LED light at several spectral regions is used for illumination, and round skin spot of diameter 34 mm or 11 mm is imaged by a CMOS sensor via cross-oriented polarizing filter. Four consecutive imaging series are performed: (1) RGB image at white LED illumination for revealing subcutaneous structures; (2) four spectral images at narrowband LED illumination (450, 540, 660, and 940 nm) for mapping of the main skin chromophores and diagnostic indices; (3) video-imaging under green LED illumination for mapping of skin blood perfusion; (4) autofluorescence video-imaging under UV (365 nm) LED irradiation for mapping of the skin fluorophores. Design details of the device and its software as well as preliminary results of clinical tests are presented.
关键词： optical skin diagnostics,multimodal imaging,skin chromophore and fluorophore mapping
更新于2025-09-09 09:28:46
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Colorimetric-Fluorescent-Magnetic Nanosphere-Based Multimodal Assay Platform for Salmonella Detection
摘要： Rapid and sensitive foodborne pathogen detection assay, which can be applied in multiple fields, is essential to timely diagnosis. Herein, we proposed a multi-signal readout lateral flow immunoassay for Salmonella typhimurium (S. typhi) detection. The assay employs colorimetric-fluorescent-magnetic nanospheres (CFMNs) as labels, which possess multi-functional: target separation and enrichment, multi-signal readout and two formats of quantitation. The assay for S. typhi detection involves magnetic separation and chromatography. First, the S. typhi were separated and enriched from matrix by antibody labelled CFMNs, and then the S. typhi-containing suspension is added onto the sample pad to flow up the test strip. The introduction of magnetic separation enhances anti-interference ability and 10-fold sensitivity, making the assay possible for practical application. The assay has realized naked eye detection of 1.88×104 CFU/mL S. typhi, and 3.75×103 CFU/mL S. typhi can be detected with a magnetic assay reader, which is 2–4 orders of magnitude lower than other label-based LFIAs, with a quantitation range of 1.88×104 to 1.88×107 CFU/mL by measuring the fluorescence intensity and magnetic signal. Moreover, the successful detection of S. typhi in complex matrix (tap water, milk, fetal bovine serum and whole blood) indicated its potential application in real samples.
关键词： colorimetric-fluorescent-magnetic nanospheres,lateral flow immunoassay,Salmonella typhimurium,multimodal assay,pathogen detection
更新于2025-09-09 09:28:46
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[IEEE 2018 24th International Conference on Pattern Recognition (ICPR) - Beijing, China (2018.8.20-2018.8.24)] 2018 24th International Conference on Pattern Recognition (ICPR) - Multimodal Face Spoofing Detection via RGB-D Images
摘要： While it has been shown that using 3D information might significantly benefit face anti-spoofing systems, traditional color images are still generally used, due to several issues such as expensive hardware requirement, high time cost, or poor accessibility when obtaining and using true 3D images. Thus, we could use RGB-D images captured by relatively low cost sensors instead, e.g., Kinect cameras, to achieve better performance without consuming huge amount of time or money. This research presents a novel multimodal face anti-spoofing method, which makes full use of available information on RGB-D images and no manually chosen regions are needed. For every pair of RGB-D images, first of all, we calculate the correlation between color and depth images to detect multimodal properties; then, by analyzing the consistency of subregions extracted from the depth image, we are able to distinguish flat spoofing faces from genuine human beings. Both anti-spoofing features are fused to make final anti-spoofing decisions. Experiments on both self-collected and pubic 3DMAD datasets show that our proposed approach is effective for intra-dataset and cross-dataset testing scenarios, and that our method could deal with different presentation attacks carried by photos, tablet screens, and face masks.
关键词： presentation attack detection,depth consistency,RGB-D images,face anti-spoofing,multimodal correlation
更新于2025-09-09 09:28:46
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Multimodal imaging of foveal neovascularisation in diabetic retinopathy
摘要： Proliferative diabetic retinopathy is characterised by the growth of neovascular tissue, which almost always occurs at the optic disc and/or near the major nasal and temporal vascular arcades, sparing the foveal area.1 Finkelstein et al. ?rst described neovascular frond at the fovea arising from the perifoveal capillaries.2 A few other authors like Joondeph et al., Kurz et al., Rajagopal et al. and Seth et al. have also reported similar ?ndings.3–6 We report a rare case of unilateral foveal neovascularisation (NVF) in a middle-aged (40 years) woman of Asian origin who presented to us with chief complaints of blurring of vision in both eyes (left more than right) over the past few weeks, which was sudden in onset and progressive in nature. The patient had type 2 diabetes mellitus and dyslipidaemia for the past nine years. Her HbA1c was 10.5 per cent. On examination, her best-corrected visual acuity was 6/12 in the right eye and 6/24 in the left eye. The anterior segment was within normal limits for her age. Meticulous examination of the iris did not reveal any sign of neovascularisation. Posterior segment examination revealed proliferative diabetic retinopathy changes in both eyes with vitreous haemorrhage in the left eye only. The right eye was noted to have a whitish tuft at the fovea on biomicroscopy which turned out to be vascular in nature with distorted foveal avascular zone (FAZ) in both multicolor (Figure 1) and re?ectance (Figure 2) image. This was con?rmed with fundus ?uorescein angiography (confocal scanning laser ophthalmoscope-based; Figure 3) and spectral domain optical coherence tomography (Figure 4). Unlike previous reports, except for the recent report by Seth et al.,6 this patient had non-insulin-dependent diabetes mellitus. It is possible that this type of diabetes mellitus is not a predictor of NVF, but rather compromised ocular blood ?ow and duration and control of diabetes are predictors as reported by Summanen et al.7 In concordance with Finkelstein et al.,2 Joondeph et al.3 and Seth et al.,6 we noted macular ischaemia in our case, whereas Kurz et al.4 and Rajagopal et al.5 reported cases with perfused macula. Our case had unilateral NVF as reported by Joondeph et al.,3 Kurz et al.4 and Seth et al.,6 whereas Finkelstein et al.2 and Kurz et al.4 reported bilateral cases. We report for the ?rst time multimodal imaging of foveal neovascularisation in diabetic retinopathy. Limitations include a lack of early-phase fundus ?uorescein angiography imaging, green re?ectance, and optical coherence tomography angiography images. The early phase of ?uorescein angiography would have helped us with more effective visualisation of distorted FAZ and leakage from the NVF. Green re?ectance utilises a longer wavelength as compared to blue and has less absorption by the crystalline lens and xanthophyll pigments (at fovea) and therefore better delineates the fovea. In our case, this would have been the better imaging modality than blue re?ectance. Optical coherence tomography angiography beyond being non-invasive would have helped us to portray the FAZ and origin of foveal neovascularisation in a more desirable way, which we lacked.
关键词： multimodal imaging,diabetic retinopathy,foveal neovascularisation
更新于2025-09-09 09:28:46
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An Innovative Approach for Person Identification by Detection and Extraction of Optic Disc from Retina and Concha from Ear
摘要： Person identification based on unimodal biometric system suffers from intra class similarity, non universality, distinctiveness and spoof attacks. To alleviate the problem faced in unimodal biometric system biometric traits are combined in multimodal biometric system. In this study a new approach, to improve the recognition rate, reduces computational complexity and storage space is presented. Distinct method of person identification using detection and extraction of optic disc from retina and concha from ear is carried out Region Of Interest (ROI) locator which is proposed here automatically detects the optic disc either from right or left eye and extracts it. Feature level fusion of optic disc and concha is done for recognition of a person. The method is tested with ROI locator and without ROI locator on publicly available databases and the experimental result shows that our multimodal biometric system outperforms with ROI locator than Without ROI locator. Matching Rate (MR) of 95 to 100% and Equal Error Rate (EER) of less than 10% is achieved with this system. The new approach was tested for unimodal system with ROI locator and was able to achieve 100% Matching Rate.
关键词： Optic Disc,Feature Level Fusion,Multimodal Biometric System,Unimodal Biometric System,Concha
更新于2025-09-04 15:30:14
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Multimodal Imaging in Lung Cancer: It Is Time to Change
摘要： The imaging techniques can be classified into two main groups: Structural/morphological imaging (SMI), which includes X rays (XR), computed tomography (CT), ultrasounds (US) as well as some varieties of magnetic resonance (MRI), and shows anatomic-morphological aspects, and molecular imaging (MI), which includes nuclear medicine (SPECT, PET), fMRI, optical and nanosystems techniques, and provides information about biochemistry/biological activity, often before structural changes. According to Society of Nuclear Medicine and Molecular Imaging, MI “is the visualization, characterization, and measurement of biological processes at the molecular and cellular levels in humans and other living systems”. MI procedures are noninvasive, safe and painless. Its sensitivity is greater than SMI, but it lacks anatomical detail, which has led to the development of multimodal imaging, combining structural and molecular techniques, widely used at present in daily practice. The pillars of MI are biochemistry/biology, instrumentation and software, and its cycle is the following: study of biology/biochemistry of a process, establishment/definition of specific targets, and development of tracers, preclinical imaging, histological validation and finally clinical imaging. This new concept led to the individualized diagnostic and treatment, being the patient the center of the medical activity. “As opposed to the doctor-centric, curative model of the past, the future is going to be patient-centric and proactive” said Dr. Zerhouni (NIH Medline Plus Winter 2007). The doctor must adapt to the needs of the own patient and this fact requires a true change of heart, because MI is intimately tied to the biology of the disease to analyzing. A new and strong interrelationship came into being: a bidirectional system biology-imaging that will allow to be much more effective in the daily practice, not only in relation to diagnosis (specific and early), but also with therapy (guide cancer treatment selection and evaluate early treatment response). There is an absolute necessity to lock the two together. Likewise, in the future the biology of a disease will indicate us what is the most adequate imaging technique and vice versa. In this regard, we know that in non-small cell lung cancer (NSCLC), ALK+ status is associated with distinct characteristics at CT imaging (CT radiogenomic characterization) [1], and that in lung adenocarcinomas 18F-FDG uptake values are related with expression levels of cellular Glucose Transporters and EGFR mutations. For this reason, different EGFR mutations correlate with different FDG uptake values.
关键词： Small Cell Lung Cancer,PET,Biomarkers,CT,MRI,Lung Cancer,Molecular Imaging,Multimodal Imaging,Non-Small Cell Lung Cancer,Structural/Morphological Imaging
更新于2025-09-04 15:30:14
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A brief review on mass/optical spectrometry for imaging analysis of biological samples
摘要： Imaging analysis, especially bioimaging analysis, has been a hot research topic in recent years. There are numerous imaging analysis techniques for diverse applications of a wide spectrum of samples, with their unique advantages and disadvantages, and there are several related reviews published yearly. Among them, imaging mass spectrometry (IMS) technique for studying the distribution of molecular or ionic species at the level of tissue, cell, or subcellular, with its main feature of combining mass spectra for molecular identification and image visualization for quick and convenient analysis. The IMS does not require chemical labeling or complex sample preparation. This review, mainly focuses on the popular emerging IMS technique, including related ionization techniques in connection with their IMS applications, and some unique optical imaging techniques such as chemiluminescence imaging and dual-modal bioimaging for biological sample analysis, with 105 related recent references.
关键词： laser,multimodal bioimaging,biomedical imaging,chemiluminescence,optical spectrometry,bioimaging analysis,dual-modal bioimaging,mass spectrometry,Imaging analysis,ionization source
更新于2025-09-04 15:30:14