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Rapid quality control of medicine and food dual purpose plant polysaccharides by matrix assisted laser desorption/ionization mass spectrometry
摘要： With the multiple biological activities and health benefit effects, polysaccharide in medicine and food dual purpose plants (MFDPPP) has been extensively applied in many fields, such as medical treatment, stock farming, and cosmetic industry. However, up to date, quality issues of MFDPPP and analytical technologies of polysaccharide post challenges to chemists. Reported herein is a rapid and high-throughput quality control method for analyzing MFDPPP, based on the matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). For the analysis of illegal addition and doping, ferroferric oxide nanoparticles was screened out as MALDI matrix to avoid the small molecule interference. Qualitatively, high sensitivity was obtained for both illegal drugs and glucose. Quantitatively, the best linear response (R2 > 0.99) was attained at the concentration range from 0.005 to 1 mg·mL-1 for glucose as an example. For the analysis of polysaccharides, 2,5-dihydroxybenzoic acid/N-methylaniline was employed as MALDI matrix to increase the detection sensitivity and mass range coverage. Furthermore, the established method was successfully applied to the analysis of supplements from Astragalus polysaccharides and Lentinan real samples, respectively, thus showing its potential in quality control for MFDPPP.
关键词： medicine and food dual purpose plants,MALDI-MS,illegal adulterations,quality control,polysaccharides
更新于2025-09-23 15:19:57
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Hybrid quantum dot-based theranostic nanomedicines for tumor-targeted drug delivery and cancer imaging
摘要： Quantum dots (QDs) are considered one of the most efficient tools used in theranostic applications for diagnosis and therapy due to their unique physiochemical characteristics. QDs are semiconductor crystals in the nano-scale range of 2–10 nm which exhibit unique photoluminescence characteristics as well as electronic properties such as tunable emission from visible to near infrared wavelengths and superior light stability. QDs possess strong photoluminescence with high molar extinction coefficient values, which make them the best candidates for cell labeling and detection of cancer biomarkers. QDs are characterized by symmetric narrow-emission spectra and broad-absorption spectra. However, some fears have been raised regarding the toxicity of QDs, especially Cd-containing QDs, due to the release of Cd ions and the generation of reactive oxygen species. Therefore, strategies have been developed to reduce their toxicity and enhance their biocompatibility through hybridization with other moieties such as polymers, proteins, polysaccharides or lipids, offering efficient tumor targeting in addition to inhibiting their release into the systemic circulation. This article discusses QD-based nanohybrids for delivery of anticancer drugs in combination with cancer imaging.
关键词： lipids,proteins,nanohybrids,tumor targeting,inorganic nanoparticles,polymers,cancer theranostics,quantum dots,polysaccharides,drug delivery
更新于2025-09-19 17:15:36
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Micro-patterned cell orientation of cyanobacterial liquid-crystalline hydrogels
摘要： Control of cell extension direction is crucial for the regeneration of tissues, which are generally composed of oriented molecules. The scaffolds of highly-oriented liquid crystalline polymer chains were fabricated by casting cyanobacterial mega-saccharides, sacran, on parallel-aligned micrometer bars of polystyrene (PS). Polarized microscopy revealed that the orientation was in transverse direction to the longitudinal axes of the PS bars. Swelling behavior of the micro-patterned hydrogels was dependent on the distance between the PS bars. The mechanical properties of these scaffolds were dependent on the structural orientation; additionally, the Young’s moduli in the transverse direction were higher than those in the parallel direction to the major axes of the PS bars. Further, fibroblast L929 cells were cultivated on the oriented scaffolds to be aligned along the orientation axis. L929 cells cultured on these scaffolds exhibited uniaxial elongation.
关键词： Cell orientation,Liquid crystal hydrogels,Micropatterned scaffolds,Cyanobacteria,Sulfated polysaccharides
更新于2025-09-09 09:28:46
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Exopolysaccharide-Derived Carbon Dots for Microbial Viability Assessment
摘要： Fluorescent dye staining combined with fluorescence microscopy or flow cytometry is becoming a routine way to monitor microorganism viability that is necessary for food safety, antibiotic development, and human health. However, the conventional live/dead assay dyes suffer from high cost, inconvenient staining steps, and high cytotoxicity, which is urgently needed to overcome. Herein, cheap carbon dots, CDs-EPS605, were reported to successfully assess microbial viability in a convenient way with neglectable cytotoxicity. The fluorescent N-doped CDs-EPS605 could be facilely prepared from bacterial amino exopolysaccharide (EPS) by one-step hydrothermal carbonization, which is cost-effective and sustainable. The negatively charged CDs-EPS605 consisted of C, H, O, N, P, and S, and featured various functional groups, including -COOH, -OH, -CONH-, and -NH2. CDs-EPS605 were observed to sensitively and selectively stain dead microorganisms instead of live ones to enable discrimination of live/dead microorganisms. The labeling method with CDs-EPS605 did not require protection from light, or washing, which is convenient. Additionally, CDs-EPS605 displayed better photostability and much less cytotoxicity compared to the commercial counterpart. Altogether, CDs-EPS605 represent a simple, yet powerful staining agent for microbial viability assessment, and at the same time enrich the current applications of microbial EPS.
关键词： polysaccharides,carbon-based nanomaterial,biocompatibility,photostability,microbial viability assessment
更新于2025-09-04 15:30:14