- 标题
- 摘要
- 关键词
- 实验方案
- 产品
-
Assessing retinal ganglion cell death and neuroprotective agents using real time imaging
摘要： The evaluation of retinal ganglion cell (RGC) death is a key part of retinal disease care. Previously, we used a Sytox Orange (SO)-based real-time imaging method to assess the RGCs in mice that underwent optic nerve crush. Here, we used N-methyl-D-aspartate (NMDA) injury in rats to confirm our model and assess the effect of neuroprotective agents on RGCs. The rats received NMDA injury and the intravitreal injection of SO, a cell-impermeant dyeing compound that targets nucleic acid. After ten minutes, non-invasive confocal scanning laser ophthalmoscopy visualized damaged or dying cells. Finally, the retinas were flat-mounted for histological confirmation of RGC death, with retrograde Fluorogold labeling and Alexa Fluor 488 Annexin V-conjugate (Annexin V) staining. This also revealed the time course of retinal cell death and the neuroprotective effect of SNJ-1945. Real-time imaging showed that SO-positive cells significantly increased starting 2 hours after NMDA injection and reached an approximate plateau at 3 hours. SO-positive cells were positive for Fluorogold and Annexin V in the isolated retinas. Moreover, the number of SO-positive retinal cells was significantly lower after treatment with SNJ-1945, compared to carboxymethyl cellulose. These results were confirmed in the isolated retinas. Thus, real-time imaging with SO allows the quick quantification of NMDA-induced RGC damage and death, and evaluation of neuroprotective agents. This technique may aid research into the development of new neuroprotective therapies.
关键词： retinal ganglion cell,Real-time imaging,SYTOX orange,neuroprotection
更新于2025-11-21 11:24:58
-
Zwitterionic near-infrared fluorophore-conjugated epidermal growth factor for fast, real-time, and target-cell-specific cancer imaging
摘要： Epidermal growth factor receptor (EGFR) is overexpressed in many types of cancers, which is associated with metastatic potential and poor prognosis in cancer patients. Therefore, development of EGFR-targeted sensitive imaging probes has been a challenge in tumor targeting, image-guided cancer surgery, patient-selective anti-EGFR therapy, and efficient targeted therapies. Methods: We synthesized a zwitterionic near-infrared fluorophore (ATTO655)-conjugated epidermal growth factor (EGF) as a novel activatable molecular probe. Fluorescence OFF/ON property and EGFR-targeting specificity of EGF-ATTO655 as well as its utility in real-time near-infrared (NIR) fluorescence imaging of EGFR-positive cancers were evaluated using in vitro and in vivo studies. Results: When conjugated to EGF, the fluorescence of ATTO655 quenched efficiently by photo-induced electron transfer (PET) mechanism between the conjugated dyes and nearby amino acid quenchers (tryptophan/tyrosine residues), which was stably maintained at physiological pH and in the presence of serum for at least 17 h. The fluorescence of EGF-ATTO655 turned on by receptor-mediated endocytosis and subsequent disintegration of EGF in EGFR-positive A431 cancer cells, thereby enabling specific and real-time fluorescence imaging of EGFR-positive cancer cells. Consequently, EGFR-positive tumors could be clearly visualized 3 h post-injection with a significantly high tumor-to-background ratio (TBR = 6.37). Conclusion: This PET mechanism-based OFF/ON type of EGF probe showed great potential for rapid, real-time, and target-cell-specific imaging of EGFR-overexpressing cancers in vitro and in vivo.
关键词： photo-induced electron transfer,Epidermal growth factor,real-time cancer imaging,tumor-specific targeting
更新于2025-11-21 11:24:58
-
Enhancement of objects in photoacoustic tomography using selective filtering
摘要： Here we developed a real-time photoacoustic tomography (PAT) imaging acquisition device based on the linear array transducer utilized on ultrasonic devices. Also, we produced a phantom including diverse contrast media and acquired PAT imaging as the light source wavelength was changing to see if the contrast media reacted. Indocyanine green showed the highest reaction around the 800-nm band, methylene blue demonstrated the same in the 750-nm band, and gold nanoparticle showed the same in the 700-nm band. However, in the case of superparamagnetic iron oxide, we observed not reaction within the wavelength bands used herein to obtain imaging. Moreover, we applied selective filtering to the acquired PAT imaging to remove noise from around and reinforce the object’s area. Consequentially, we could see the object area in the imaging was effectively detected and the image noise was removed.
关键词： Photoacoustic image,real-time,back projection algorithm,selective filtering,ultrasonic imaging
更新于2025-09-23 15:23:52
-
Experimental verification of turbidity tolerance of stereo-vision-based 3D pose estimation system
摘要： This paper presents the verification of the turbidity tolerance of a stereo-vision-based 3D pose estimation system for underwater docking applications. To the best of the authors’ knowledge, no studies have yet been conducted on 3D pose (position and orientation) estimation against turbidity for underwater vehicles. Therefore, the effect of turbidity on the 3D pose estimation performance of underwater vehicles and a method of operating under turbid conditions were studied in this work. A 3D pose estimation method using the real-time multi-step genetic algorithm (RM-GA) proposed by the authors in the previous works shows robust pose estimation performance against changing environmental conditions. This paper discusses how and why the RM-GA is well suited to effective 3D pose estimation, even when turbid conditions disturb visual servoing. The experimental results confirm the performance of the proposed 3D pose estimation system under different levels of turbidity. To demonstrate the practical usefulness of the RM-GA, docking experiments were conducted in a turbid pool and a real sea environment to verify the performance and tolerance of the proposed system under turbid conditions. The experimental results verify the robustness of the system against turbidity, presenting a possible solution to a major problem in the field of robotics.
关键词： Robustness against turbidity,Real-time multi-step genetic algorithm,Sea docking,3D pose estimation,Stereo-vision,Visual servoing
更新于2025-09-23 15:23:52
-
Real-time detection of moving cast shadows using foreground luminance statistics
摘要： This paper presents a robust real-time method for detection of moving cast shadows which employs the assumption of higher interdependence of luminance values for the shadow pixels in larger regions compared to the object pixels. First, a fast modified image differencing technique is used to separate foreground pixels from the background. Next, for a moving window of fixed width scanning the foreground regions, a new measure called Modified Correlation is introduced. The new measure is determined by first computing the correlation between the luminance values of the moving window and luminance values of its neighbouring windows; this correlation is then divided by a robust-to-noise range measured based on the luminance values of the moving window. The modified correlation exhibits abrupt rising transitions as it enters the shadow region from the object region, transitions which can be used to separate object pixels from shadow pixels. Thus, the new method is very effective at suppressing moving cast shadows, while avoiding limiting structures, unrealistic assumptions, the need for a-priori knowledge, and manual selection of critical parameters. An average shadow detection rate of 85.4% and an average shadow discrimination rate of 99.5% over multiple different sequences, higher than those of available methods in the literature, confirm the efficacy of the method. The robustness of the method is examined under different lighting conditions, different target-environment combinations, and sequences with object-shadow occlusion. The proposed method is computationally efficient and suitable for real-time situations.
关键词： Moving cast shadow,Image difference,Correlation,Sliding window,Foreground mask,Real-time detection
更新于2025-09-23 15:23:52
-
Real-time adaptive optical self-interference cancellation system for in-band full-duplex transmission
摘要： A real-time adaptive optical self-interference cancellation (RTA-OSIC) system is proposed and demonstrated for in-band full-duplex transmission. The RTA-OSIC system is controlled automatically by adaptive algorithm running on a real-time microcontroller STM32. Modified Hooke-Jeeves (MHJ) algorithm is proposed to search the optimal optical parameters more rapidly. Experimental results show that the MHJ algorithm requires 15-25 samples to find the optimal point. The RTA-OSIC system achieves more than 22 dB cancellation depth within 0-700MHz frequency band. Compared with the existing adaptive OSIC system which uses the Nelder–Mead Simplex algorithm, there are 60% less samples for our RTA-OSIC system with MHJ algorithm.
关键词： self-interference cancellation,Modified Hooke-Jeeves (MHJ) algorithm,microwave photonics,real-time adaptation
更新于2025-09-23 15:23:52
-
Numerical investigation of energy performance and cost analysis of Moroccan’s building smart walls integrating vanadium dioxide
摘要： Thermochromic materials such as vanadium dioxide (VO2) have the capability to regulate their infrared reflectivity depending on ambient temperature. Such smart materials are attractive for applications like smart windows and smart roofs. In this paper, we investigate numerically the impact of tungsten (W) doped VO2 application as a smart outdoor wall layer on the building energy consumption in the Mediterranean climate. Temperature distributions through wall configurations with and without W doped VO2 were calculated using finite differences method implemented in Matlab environment. Calculations take into consideration dynamic variations of W doped VO2 absorptivity depending on outdoor temperature variations. Decrement factor, time lag, transmission and peak loads of cooling and heating were calculated. Results have shown that the indoor surface temperature is attenuated during summer by 2–3 °C depending on orientation. Cooling energy saving due to the use of W doped VO2 in summer is around 70% monthly which is equivalent to save $21.6/month of electricity invoiced amount. During winter, W doped VO2 maintains the same value of absorptivity as the uninsulated wall. Thus, results show small differences for indoor surface temperature and heating energy consumption. The application of W doped VO2 in intelligent thermal wall insulation offers a real-time dynamic variation of building’s envelope solar absorptivity and makes smart walls concept more feasible.
关键词： Energy efficiency,Smart wall,Radiative thermal rectification,Thermochromic,Real-time dynamic absorptivity,Vanadium dioxide
更新于2025-09-23 15:23:52
-
Automatic bathymetry retrieval from SAR images
摘要： Bathymetry, the topography of the sea floor, is in high demand due to the increase in offshore constructions like wind parks. It is also an important dataset for climate change modelling, when sea level rises and changes in circulation currents are to be simulated. The retrieval of accurate bathymetry data is a cost-intensive task usually requiring a survey vessel charting the respective area. However, bathymetry can also be retrieved remotely using data from Earth observation satellites. The main point of this study is the development of a processor that allows the automatic derivation of gridded bathymetry information from spaceborne Synthetic Aperture Radar (SAR) data. Observations of sea state modifications in SAR images are used to derive the bathymetry in shelf areas using the shoaling effect, which causes wavelengths to become shorter when reaching shallower waters. The water depth is derived using the dispersion relation for surface water waves, which requires wavelength and wave period as input parameters. While the wavelength can be directly retrieved from the SAR image, for the peak period additional information and procedures are required, e.g. local measurements or complex SAR data. A method for automatically deriving the wave period for swell waves in SAR images was developed and tested in this paper. It uses depth data from public databases as initial values which are compared to derived depths iterating through possible peak periods along the calculation grid; the peak period resulting in a minimal root-mean-square deviation is then used for bathymetry calculation. The bathymetry derived from a TerraSAR-X acquisition of the Channel Islands is presented; the resulting peak wave period of 11.3 s fits well to nearby in situ measurement data.
关键词： Bathymetry,Remote sensing,Near-real time processing,Synthetic aperture radar
更新于2025-09-23 15:23:52
-
New approach to enhancing the performance of cloud-based vision system of mobile robots
摘要： Mobile robots require real-time performance, high computation power, and a shared computing environment. Although cloud computing offers computation power, it may adversely affect real-time performance owing to network lag. The main objective of this study is to allow a mobile robot vision system to reliably achieve real-time constraints using cloud computing. A human cloud mobile robot architecture is proposed as well as a data flow mechanism organized on both the mobile robot and the cloud server sides. Two algorithms are proposed: (i) A real-time image clustering algorithm, applied on the mobile robot side, and (ii) A modified growing neural gas algorithm, applied on the cloud server side. The experimental results demonstrate that there is a 25% to 45% enhancement in the total response time, depending on the communication bandwidth and image resolution. Moreover, better performance in terms of data size, path planning time, and accuracy is demonstrated over other state-of-the-art techniques.
关键词： Computation offloading,Computer vision,3D point cloud,Mobile robot,Stereo vision,Real-time networking,Cloud computing,Cloud robotics
更新于2025-09-23 15:23:52
-
[IEEE 2018 IEEE International Conference on Signal Processing, Communications and Computing (ICSPCC) - Qingdao, China (2018.9.14-2018.9.16)] 2018 IEEE International Conference on Signal Processing, Communications and Computing (ICSPCC) - A Real-time Detection Algorithm for Unmanned Aerial Vehicle Target in Infrared Search System
摘要： Aiming at the difficulty of infrared target detection of 'low and slow small' unmanned aerial vehicles (UAV) in complex low-altitude background, this paper proposes a new target detection algorithm based on multiscale fusion filtering. Combined with spatial multiscale decomposition filtering and temporal multiscale difference processing, the algorithm can effectively overcome many difficulties such as complex low-altitude background interference, unknown target scale, unknown angular velocity and low target signal-to-noise ratio (SNR). The test result shows that the algorithm can effectively detect the UAV targets with different distances in complex low-altitude background, and the false alarm rate is low. The algorithm is realized in TI 6657 DSP and realizes 100Hz real-time processing of mid-wave infrared images with 640*512 resolution, which has been effectively applied to the large-field circumferential scanning infrared search system developed by ATR Lab.
关键词： real-time algorithm,multiscale fusion filtering,UAV target detection,low-altitude background,low and slow small targets
更新于2025-09-23 15:23:52