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oe1(光电查) - 科学论文

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  • Fluorescence and absorption studies of DNA-Pd(II) complex interaction: Synthesis, spectroanalytical investigations and biological activities

    摘要： Novel palladium(II) complexes (7a–7e) of substituted quinoline derivatives were synthesized. The complexes were characterized using various techniques such as thermogravimetric analysis (TGA), elemental analysis, conductance measurement, mass, absorption, infra‐red (IR), 1H NMR, 13C NMR and energy‐dispersive X‐ray spectroscopy (EDX). Complexes for herring sperm DNA (HS DNA) binding were explored and absorption titration and the binding constant (Kb) as well as Gibb's free energy were evaluated. Complex 7d exhibited the highest binding constant, therefore the thermodynamic parameters of 7d at different temperatures were evaluated. To support the results of the absorption titration, fluorescence titration, viscosity measurement and molecular docking studies were performed. The fluorescence quenching data as evaluated from Stern–Volmer equation were used to calculate KSV, K f and the number of binding sites. The results of all these studies were in good agreement with the absorption study. DNA electrophoretic mobility was performed to explore the possible application of metal complexes as artificial metallonucleases. The antibacterial activity of the complexes was accessed against different pathogenic bacteria and cytotoxicity was measured using brine shrimp and S. pombe.

    关键词： Stern–Volmer equation,absorption titration,thermodynamic parameters,cytotoxicity,fluorescence quenching,artificial metallonuclease

    更新于2025-09-23 15:23:52

  • Fluorescence Spectral Study on the Interaction Between Brilliant Blue and Trypsin

    摘要： The interaction between brilliant blue and trypsin was studied by using fluorescent technique. The fluorescence quenching data were analyzed according to Stern-Volmer equation and Line weaver-Burk equation. The binding constants, thermodynamic parameters and binding sites were obtained. According to the thermodynamic parameters, the main binding force between brilliant blue and trypsin was ascertained as electrostatic interaction. The binding distance between brilliant blue and trypsin was also given based on the F?rster energy transfer theory.

    关键词： Fluorescence spectrum,Thermodynamic parameters,Trypsin,Brilliant blue

    更新于2025-09-09 09:28:46