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Black phosphorus nanosheets based sensitive protease detection and inhibitor screening
摘要： Proteases, as one of the most significant kind of digestive enzymes, are closely related to a variety of physiological processes and diseases. Herein, a black phosphorus nanosheets (BPs) based sensitive fluorometric method for protease detection and inhibitor screening is proposed. The aqueous solution of perylene probe (probe 1) displays a strong fluorescence. BPs, as novel discovered two-dimensional (2D) materials, can adsorb probe 1 through electrostatic interactions, which causes fluorescence quenching of probe 1. Histone can control the interactions between the perylene probe and BPs, which can be further regulated by the introduction of a protease. Thus, the protease activity can be monitored by detecting the fluorescence intensity changes of probe 1. The method is label free, sensitive and selective. As low as 1 ng/mL trypsin can be easily detected.
关键词： trypsin,inhibitor,protease,black phosphorus nanosheets,fluorescence,perylene probe
更新于2025-09-23 15:23:52
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Aprotinin Encapsulated Gold Nanoclusters: A Fluorescent Bioprobe with Dynamically Nuclear Targeting and Selective Detection of Trypsin and Heavy Metal
摘要： Fluorescence imaging has currently emerged as one of the most frequently used noninvasive imaging technology to selectively monitor biological processes in living systems. In past decades, gold nanoclusters (Au NCs) received increasing attraction because of their intrinsic fluorescence and their inherent biocompatibility. As a stabilizing and reducing agent, an abundant, sustainable and widely used polypeptide derived drug molecule, aprotinin (Ap) is selected for the synthesis of Au nanoclusters (Ap-Au NCs) due to characteristic bioactivity, excellent biocompatibility, biodegradability, nonallergenic character. Herein, Ap encapsulated Au NCs exhibiting desirable red fluorescence feature was facilely produced for the first time, which were subsequently used for cell imaging and detection of various analytes. Much interestingly, dynamically subcellular localization of Ap-Au NCs from the cytoplasm to the nucleus in Hela cells was observed. Afterward, it has shown the selective and quantitative detection of trypsin by using Ap encapsulated Au NCs. Lastly, Ap-Au NCs were readily used for detection of mercury and copper quantitatively. The photoluminescence of the Ap-Au NCs was quenched with the addition of metioned analytes. This study opens crucial insights on the integration of biomolecule with metal nanoclusters, also discusses a multifunctional nanomaterial platform for cell imaging, subcellular targeting, biosensing and drug delivery.
关键词： trypsin detection,gold nanoclusters,aprotinin,cell imaging,heavy metal detection,Fluorescence imaging
更新于2025-09-23 15:21:01
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ε-Poly-L-lysine-protected Ti3C2 MXene quantum dots with high quantum yield for fluorometric determination of cytochrome c and trypsin
摘要： Titanium carbide quantum dots functionalized with ε-poly-L-lysine (PLL) were synthesized by sonication cutting and hydrothermal synthesis. The deprotonated Ti3C2 MXene quantum dots (Ti3C2 MQDs) exhibit excitation wavelength-dependent blue photoluminescence with typical excitation/emission peaks at 330/415 nm and a quantum yield of 22% due to strong quantum confinement. The fluorescence of ε-poly-L-lysine protected Ti3C2 MQDs (PLL-protected Ti3C2 MQDs) is reduced via an inner filter effect after the addition of cytochrome c (cyt-c). Response to cyt-c is linear in the 0.2 to 40 μM concentration range and the detection limit is 20.5 nM. In the presence of trypsin, cyt-c is hydrolyzed to small peptides, and the Fe3+ ion in cyt-c probably is reduced to Fe2+ with the aid of the digestive enzyme. This results in the restoration of the blue fluorescence of the modified MQDs. Fluorescence increases linearly in the 0.5 to 80 μg mL?1 trypsin concentration range with the detection limit of 0.1 μg mL?1. The method was successfully applied to the determination of cyt-c and trypsin in spiked serum samples.
关键词： Hydrothermal method,Inner filter effect,Fluorescence,Trypsin,Cytochrome c,Ti3C2 MXene quantum dots
更新于2025-09-12 10:27:22
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A facile method toward potentially next-generation bacteria detectors using polymer/MWCNT/Au nanocomposite films: A possibility to detecting ability through the shift in resonance frequency
摘要： The global market demands for new disposable wireless sensors for food, clinical, tissue, and drug industries in the near future. Gelatin-based multi-layer films were successfully prepared using a room temperature solution casting method. Anticipating future developments, we have looked at the potential applications of cost-effective passive RFID tags in the detection of bacteria (here trypsin was used for biosensing tests) in body fluids, foods, medicines, and much more. This paper is concerned with the measurement of the dielectric constant of materials remotely by using the frequency shift of RFID tags, in the first step. In continue, we establish a setup along with a novel multi-layer structure of gelatin films determining whether there exists any bacterium in a biological fluid or not, by using a physical approach. For this, penetration of bacterium through gelatin multi-layer films causes the RFID tags to be exposed to the biological fluid changing the dielectric constant, detected as a frequency shift. Samples were characterized utilizing FTIR, XRD, TGA, FESEM, tensile, and VNA analyzes. Our proposed wireless biomimetic sensor is able to detect trypsin within a few minutes. The immediate application of our designed biosensor is for the elderly and infants diapers, but further applications in biomedicine, biomedical engineering, food industry, and things like that can be anticipated.
关键词： Gelatin,Vector Network Analyzer,Au Nanoparticles,Trypsin,Carbon nanotubes
更新于2025-09-10 09:29:36
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Sensitive fluorescence sensor for point-of-care detection of trypsin using glutathione-stabilized gold nanoclusters
摘要： There is an urgent demand for on-site detection of biomarker, particularly in clinical diagnosis and therapeutics applications. Herein, we designed a convenient and sensitive fluorescence nanosensor for point-of-care determination of trypsin (TRY). The fluorescence intensity of gold nanoclusters (AuNCs) can be quenched by cytochrome c (Cyt c) via electron transfer mechanism. TRY specifically catalyzes the hydrolysis of Cyt c to produce small peptide fragments, inducing the significant fluorescence recovery. As a result, the AuNCs-based system processed a sensitive and selective response to TRY with the range 0.2-100 μg mL-1, accompanying a detection limit of 0.08 μg mL-1. Significantly, the sensing assay can be used to construct test strips for rapid and visual recognition of TRY. Combining with smartphone and ImageJ software, we further developed an image processing algorithm for quantitative detection of TRY with highly promising, which validated the potential point-of-care application.
关键词： Gold nanoclusters,Fluorescence,Electron transfer,Trypsin,Test strips
更新于2025-09-10 09:29:36
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Fluorescence Spectral Study on the Interaction Between Brilliant Blue and Trypsin
摘要： The interaction between brilliant blue and trypsin was studied by using fluorescent technique. The fluorescence quenching data were analyzed according to Stern-Volmer equation and Line weaver-Burk equation. The binding constants, thermodynamic parameters and binding sites were obtained. According to the thermodynamic parameters, the main binding force between brilliant blue and trypsin was ascertained as electrostatic interaction. The binding distance between brilliant blue and trypsin was also given based on the F?rster energy transfer theory.
关键词： Fluorescence spectrum,Thermodynamic parameters,Trypsin,Brilliant blue
更新于2025-09-09 09:28:46