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[IEEE 2018 43rd International Conference on Infrared, Millimeter, and Terahertz Waves (IRMMW-THz2018) - Nagoya, Japan (2018.9.9-2018.9.14)] 2018 43rd International Conference on Infrared, Millimeter, and Terahertz Waves (IRMMW-THz) - Detection of Human Tumor Markers with THz Metamaterials
摘要： THz bioanalytical techniques are already proven as a viable tool for the label-free detection of biomolecules. But still, significant improvement of THz sensing techniques is needed to reach application relevant sensitivity levels. We present the label-free THz sensing of human tumor markers using a highly sensitive metamaterial based THz biosensor. High affinity binding of the specific protein to DNA is utilized for localized functionalization to further enhance the detection sensitivity.
关键词： tumor markers,DNA-protein interaction,THz sensing,metamaterials,biosensor
更新于2025-09-23 15:21:01
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Enhanced light-emitting diode induced fluorescence detection system with capillary electrophoresis
摘要： An enhanced fluorescence detection system of capillary electrophoresis (CE) was equipped with a concave silver mirror, by which the detection sensitivity of light-emitting diode induced fluorescence (LEDIF) can be increased greatly. The silver concave mirror and the cathode window in photomultiplier tube (PMT) were accurately set face to face at the same axis. When the two labeled tumor markers exactly moved to the center of detection window, the emission from analytes are excitated by LED source. Currently, the analytes may be regarded as a luminescent source point. When the source point exactly moves to the focus of the concave mirror, the emission of the labeled sample was collected effectively, enhanced by convergence and reflected by the concave mirror. Then it was sensitively detected by the PMT. The optical mechanism of enhancing detection sensitivity was explored. A simple comparative test on sensitivity was carried out, which aimed to compare sensitivity of the new detection system with concave mirror to that without concave mirror but the other conditions were kept the same. Two tumor markers labeled with FITC were selected for the test, using the simple LEDIF detect system. The results (LOD, 150 nM for L-Leu and L-Val) showed that the detection sensitivity matched with concave mirror reached more 16 times than the detection method without concave mirror.
关键词： Curvature radius,Light-emitting diode induced fluorescence,Tumor markers,Concave silver mirror,Polarized light,Highly sensitive optical detector
更新于2025-09-19 17:13:59
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Gold nanoparticles – an optical biosensor for RNA quantification for cancer and neurologic disorders diagnosis
摘要： The objective of this study is to develop a facile tool for the absolute detection and quantification of nucleic acid transcripts, using a gold nanoparticle-based optical biosensor. Topoisomerase 1 (TOP1) and tyrosyl DNA phosphodiesterase 2 (TDP2) were among the nucleic acid transcripts of choice due to their role as genomic instability biomarkers and their implication in various cancers and neurologic disorders. This opens the door to develop a simple tool that can be used for diagnosing and monitoring treatment response for such diseases, overcoming the requirements for high cost, time, and complexity of the existing technologies for the absolute quantification of transcripts of interest.
关键词： RNA extraction,genomic instability,tumor markers,gold aggregating gold,TDP2,TOP1,neurodegenerative diseases,magnetic nanoparticles
更新于2025-09-04 15:30:14