研究目的
To contribute to the information on the nutritional value of widely consumed tea infusions, by determining the vitamin B2 content of tea plants using the CE-LIF method. Moreover, the correlation between the vitamin B2 content of tea plants with their total phenolics and antioxidant capacity are evaluated in this study.
研究成果
A fast, simple, and sensitive CE-LIF system was used to determine the riboflavin (vitamin B2) content of 14 tea infusions including black, green, sage, and rosemary teas. The RF content of all tea samples suggests that tea infusions are amongst important dietary sources of RF for prevention of diseases caused by vitamin B2 deficiency.
研究不足
The study did not explore the long-term stability of RF in tea infusions beyond the 5-minute incubation period. The sensitivity of RF to light could also affect the accuracy of the measurements if not properly controlled.
1：Experimental Design and Method Selection:
The study used a capillary electrophoresis method combined with laser-induced fluorescence detection for the sensitive detection of vitamin B2 in tea infusions.
2：Sample Selection and Data Sources:
Four black and two green tea-bag samples were obtained from local markets in Istanbul, and sage and rosemary samples were purchased from Istanbul and Boston markets.
3：List of Experimental Equipment and Materials:
An Agilent capillary electrophoresis system equipped with a ZETALIF 2000 LIF detector was used. The separation was performed at 25 kV, and the temperature was set at 25 °C.
4：Experimental Procedures and Operational Workflow:
Tea leaves were placed in boiling water and incubated in a water bath for 5 min. After filtration, tea infusions were directly injected into the capillary column.
5：Data Analysis Methods:
The data processing was carried out with the Agilent ChemStation software.
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