研究目的
To synthesize size-controlled, time-efficient silver nanoparticles (AgNPs) from Pleurotus florida using ultra-violet, visible range, and microwave radiations and assess their antibacterial activity.
研究成果
Microwave-assisted synthesis was found to be the most efficient method for producing uniform and small-sized AgNPs. The synthesized nanoparticles exhibited significant bactericidal effects on various gram-positive and gram-negative bacteria, indicating their potential for use in antibacterial materials.
研究不足
The study highlights the variability in size and uniformity of AgNPs synthesized using different radiations, with UV and visible light producing less uniform and larger particles compared to microwave radiation. The antibacterial activity was not observed against E. coli, possibly due to the larger size of the nanoparticles.
1：Experimental Design and Method Selection:
Synthesis of AgNPs was performed by irradiating Pleurotus florida cell free extract mixed with silver nitrate solution using microwave, visible light, and ultra-violet radiations for various intervals of time.
2：Sample Selection and Data Sources:
Fruit body of P. florida was used to prepare cell free extract. Bacterial isolates were procured from Department of Microbiology, Punjab Agricultural University, Ludhiana and Christian Medical College and Hospital, Ludhiana.
3：List of Experimental Equipment and Materials:
Elico SL 218 Double Beam UV–vis Spectrophotometer, Hitachi H-7650 TEM, Nicolet 6700 FTIR, Beckman Coulter DelsaTM Nano HC Particle Analyzer.
4：Experimental Procedures and Operational Workflow:
The synthesis process involved irradiation of the mixture with different radiations, followed by characterization using UV–vis spectroscopy, TEM, FTIR, and DLS. Antibacterial assay was conducted using well diffusion method.
5：Data Analysis Methods:
UV–vis spectra were analyzed for surface plasmon resonance peaks. TEM images were analyzed for particle size and morphology. FTIR spectra were analyzed for molecular interactions. DLS was used for hydrodynamic size distribution.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
