研究目的
Developing a new method for researching weak-UV-absorbing, non-fluorescence and nonpolar compounds based on HPLC-PR-C4D system.
研究成果
The HPLC-PR-C4D system was successfully developed for the determination of weak-UV-absorbing, non-fluorescence and nonpolar compounds, specifically CsA. The system is economical, convenient, and may be applied to other compounds. It has potential for use in pharmacokinetic studies involving humans.
研究不足
The detection limit of the proposed method is higher compared to other methods. Further study is needed to improve it.
1：Experimental Design and Method Selection:
The study utilized HPLC-PR-C4D system for the first time in analyzing weak-UV-absorbing, non-fluorescence and nonpolar compounds.
2：Sample Selection and Data Sources:
CsA was selected as the analyte. Stock solution of CsA was prepared in methanol.
3：List of Experimental Equipment and Materials:
HPLC system, low-pressure mercury lamp, PTFE and PEEK tubes, CsA capsule, health human serum, and whole blood sample were used.
4：Experimental Procedures and Operational Workflow:
The system consisted of photochemical reactor and C4D. The effect of radiation power, length of reaction tube, and thickness of detection tube were investigated.
5：4D. The effect of radiation power, length of reaction tube, and thickness of detection tube were investigated. Data Analysis Methods:
5. Data Analysis Methods: The performance of the system was evaluated by linearity, limit of detection (LOD), and repeatability.
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