研究目的
To develop a method for creating ultra-stable plasmonic colloidal aggregates for accurate and reproducible quantitative SE(R)RS in protein-rich biomedia.
研究成果
The emulsion templated method produces ultra-stable SE(R)RS active colloidal aggregates with quasi-spherical structures, offering a significant improvement over conventional methods by providing stable enhancements for days and enabling analysis in complex systems like protein-rich bio-solutions.
研究不足
The method's applicability is limited to systems where the analyte can diffuse into the hot-spots of SPAs, and the presence of high protein concentrations may slow down this process.
1：Experimental Design and Method Selection:
The study employs an emulsion templated method to form densely-packed quasi-spherical Au/Ag colloidal aggregates.
2：Sample Selection and Data Sources:
Citrate-reduced Ag and Au colloids were used.
3：List of Experimental Equipment and Materials:
Includes a Soniprep 150 Ultrasonic Disintegrator for emulsification, and chemicals like silver nitrate, tetrachloroauric (III) acid trihydrate, etc.
4：Experimental Procedures and Operational Workflow:
The colloid was emulsified with hexane and TBA+NO3-, then left for hexane evaporation to produce stable SPA colloids.
5：Data Analysis Methods:
SE(R)RS measurements were performed using a WITec Alpha 300R Confocal Raman Microscope.
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