研究目的
To investigate the dispersion time and location of the absorption of Qdot in the conjunctiva of Wistar albino rats by using QD-based biosensor probe.
研究成果
The study suggests that the conjunctival lymphatic drainage is associated with the whole body's circulatory system approximately within an hour in the rat model. Drops to the eye can reach far extremities such as the ears, forearms, hind legs, and tail.
研究不足
The study did not yield images from the nose as expected within the first 20 minutes, possibly due to limited drain or other limiting reasons such as tissue thickness or the deepness of the drainage.
1：Experimental Design and Method Selection:
The study used Qdot655 (QD), fluorescence nanoparticles, to track lymphatic drainage from the conjunctiva in Wistar albino rats. Fluorescence images were taken at different time intervals to observe the distribution of QD particles.
2：Sample Selection and Data Sources:
Five-week-old Wistar albino rats (five female and five male) were used. The rats were kept under standard conditions before and during the experiments.
3：List of Experimental Equipment and Materials:
Qdot? ITK? Carboxyl Quantum Dots (Invitrogen), Hamilton syringe (Hamilton Company), Syngene GBOX-XRQ instrument for in vivo fluorescence imaging.
4：Experimental Procedures and Operational Workflow:
A single dose of 1 μl of QD was dropped under the upper left eyelid of the rats. In vivo images were collected at the 1st, 20th, 30th, and 60th minutes following the administration.
5：Data Analysis Methods:
The strength of the observed fluorescence on the body parts of male and female rats was recorded and compared at different time intervals.
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