研究目的
Investigating the fabrication of quantum dot microstructures through a microemulsion approach for applications in immunoassays.
研究成果
The self-assembled QDs microstructures prepared by the microemulsion strategy are convenient, cheap, and easy to produce, with potential applications in biosensor, biomedicine, and biochips. The method maintains the optical and biological characters of QDs, enabling visual imaging immunoassays.
研究不足
The study focuses on water-soluble quantum dots, limiting the applicability to organic-soluble QDs without additional modification steps. The method's efficiency with other types of nanoparticles or under different environmental conditions was not explored.
1：Experimental Design and Method Selection:
The study employed a microemulsion approach to fabricate quantum dot microstructures. A polymer solution containing a stabilizer was mixed with a quantum dot aqueous solution to prepare a reversed microemulsion through shaking. The microemulsion was then cast on a solid substrate followed by evaporating steps, resulting in the formation of an ordered porous film.
2：Sample Selection and Data Sources:
The materials used included Poly(methyl methacrylate) (PMMA), polystyrene (PS), didodecylamine (DDA), N-hydroxysulfosuccinimide (NHS), PEO20-PPO70-PEO20 (P123), 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), BSA, Anti-BSA antibody (IgG), and mercaptopropionic acid modified CdTe quantum dots.
3：List of Experimental Equipment and Materials:
Scanning electron microscopy (SEM) images were collected on a JEOL JSM-6700F field emission scanning electron microscope. Confocal laser scanning microscopic (CLSM) images were obtained on an Olympus fluoview FV
4：Experimental Procedures and Operational Workflow:
10 The microemulsion was prepared by mixing PMMA and P123 dichloromethane solution with an aqueous solution of QDs, shaken for 30s, then cast onto a glass substrate under controlled humidity and temperature.
5：Data Analysis Methods:
Analysis of CLSM data was carried out using the software FV10-ASW.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容-
Polystyrene
PS
Sigma-Aldrich
Used as an alternative polymer in the preparation of the microemulsion.
-
JEOL JSM-6700F field emission scanning electron microscope
JSM-6700F
JEOL
Used for collecting SEM images of the porous film.
-
Olympus fluoview FV1000
FV1000
Olympus
Used for obtaining CLSM images of the fluorescent microstructures.
-
Poly(methyl methacrylate)
PMMA
Sigma-Aldrich
Used as a polymer in the preparation of the microemulsion for forming the porous film.
-
Didodecylamine
DDA
Aladdin
Used as a stabilizer in the preparation of the microemulsion.
-
N-hydroxysulfosuccinimide
NHS
Aladdin
Used in the preparation of the microemulsion.
-
PEO20-PPO70-PEO20
P123
Anqiushi Luxing Chemical Co. Ltd.
Used as a stabilizer in the preparation of the microemulsion.
-
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride
EDC
J&K Scientific Ltd
Used in the preparation of the microemulsion.
-
BSA
Beijing Biosynthesis Biotechnology Co. Ltd.
Used in the immunoassay experiments.
-
Anti-BSA antibody
IgG
Invitrogen
Used in the immunoassay experiments.
-
CdTe quantum dots
Used as the quantum dots in the study for fabricating microstructures.
-
登录查看剩余9件设备及参数对照表
查看全部
