摘要： In this work, we propose the preparation and investigation of advanced microfluidic Surface-enhanced Raman spectroscopy (SERS) chip with the design, allowing high SERS enhancement and analysis reproducibility. Proposed chip implements the creation of periodical metal structure (grating) inside the microfluidic chip and further immobilization of gold multibranched nanoparticles (AuMs) with shaped edges on the grating surface. Such approach allows achieving plasmonic coupling between the surface plasmon polariton wave, excited on the Au grating, and localized surface plasmon, excited on sharped edges of AuMs. As a result, the high enhancement of electric field in the space between AuMs was achieved, which results in the high SERS enhancement factor, confirmed by both, theoretical calculation and experimental measurements with typical SERS analyte – R6G. In particular, vanishingly small concentration of R6G is possible to detect using the proposed plasmonic coupling, which sensitivity significantly exceeds previously reported limits in the case of microfluidic SERS measurements. We also observed the dependency of SERS intensity on the microfluidic flow rate and demonstrated the perfect reliability of the SERS signal, measured in the microfluidic regimes under the constant flow rate.