研究目的
Investigating the differences in the fixation/internalization of three types of quantum dots by human peripheral blood neutrophilic granulocytes and lymphocytes.
研究成果
The study demonstrated that neutrophilic granulocytes and lymphocytes interact differently with quantum dots, with NG phagocytosing QD and lymphocytes fixing QD aggregations to their surface. The presence of complex functional groups on QD surfaces can inhibit their fixation/internalization by blood cells.
研究不足
The study was limited by the specific types of quantum dots used and the conditions under which the experiments were conducted. The phototoxicity of quantum dots was ruled out by conducting experiments in darkness, which may not fully represent natural conditions.
1：Experimental Design and Method Selection:
The study involved incubating heparin-treated whole blood with quantum dots or saline, followed by analysis using flow cytometry, scanning electron microscopy, and X-ray microanalysis.
2：Sample Selection and Data Sources:
Peripheral blood samples were collected from donors aged 20-40 years.
3：List of Experimental Equipment and Materials:
Equipment included a Vortex shaker (ELMI), Cytoflex S flow cytometer (Beckman Coulter), JSM-IT300LV electron microscope (Jeol), and X-MaxN 20 energy dispersion attachment (Oxford Instruments). Materials included quantum dots of three types.
4：Experimental Procedures and Operational Workflow:
Blood was incubated with quantum dots, lysed, washed, and analyzed. Lymphocyte and NG fractions were isolated, incubated with quantum dots, fixed, and scanned.
5：Data Analysis Methods:
Flow cytometry data was analyzed for fluorescence, and scanning electron microscopy and X-ray microanalysis were used for morphological and compositional analysis.
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