研究目的
To explore the molecular influence of the erbium-doped: yttrium aluminum garnet (Er:YAG) laser and cyclosporin A (CsA) on human gingival fibroblasts (HGFs) for improvement in periodontal diseases therapy.
研究成果
The results indicate that there are 2 separate biomarker panels for the 2 treatment methods, suggesting the possibility of multiple therapeutic procedures for treatment of patients. Combination of the two methods may be an effective procedure to apply in the field, but more investigations are needed about the synergic effects of the two methods.
研究不足
The study is limited by the sensitivity of the proteomic techniques used, with the number of identified proteins related to the laser effect being higher than for the drug effect due to the more sensitive method used for laser data.
1：Experimental Design and Method Selection:
Focused on articles studying the proteome profiles of HGFs after treatment with laser irradiation and application of CsA. Used Cytoscape Version
2：0 for topological features analysis of differentially expressed proteins and module selection from the PPI network using Cluster ONE plugin. Sample Selection and Data Sources:
Used PubMed database to study literature with keywords: 'gingival fibroblasts', 'Proteomics', 'drug', and 'laser therapy'. Included studies focusing on gingival tissue samples of human species and comparing drug or laser treated HGF tissues and non-treated HGF tissues.
3：List of Experimental Equipment and Materials:
Cytoscape Version
4：0, Cluster ONE plugin, PubMed database. Experimental Procedures and Operational Workflow:
Analyzed PPI networks based on topological parameters such as node degree and betweenness centrality. Performed GO enrichment analysis for densely connected regions and key proteins in both PPI networks.
5：Data Analysis Methods:
Used Cytoscape for network visualization and analysis, Cluster ONE for module selection, and ClueGO for GO enrichment analysis.
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