研究目的
Investigating the therapeutic effects of a specific herbal medicine on a particular disease.
研究成果
Quantum dots offer superior performance in simultaneous multicolor imaging of multiple biomarkers, providing insights into biomolecule interactions and disease progression. However, challenges remain in their clinical application due to toxicity and imaging limitations.
研究不足
The intrinsic toxicity of QDs, their uptake by the reticuloendothelial system, and the challenges of in vivo multicolor imaging due to weak tissue penetration of external exciting light and strong background autofluorescence.
1：Experimental Design and Method Selection:
The study discusses the synthesis of QD probes for multicolor imaging, including core-shell structure synthesis, ligand selection, and surface modification.
2：Sample Selection and Data Sources:
The research focuses on FFPE tissues or cells for imaging.
3：List of Experimental Equipment and Materials:
QDs with different emission wavelengths, antibodies, and streptavidin are used.
4：Experimental Procedures and Operational Workflow:
The study details direct and indirect staining procedures for QD-based imaging.
5：Data Analysis Methods:
Spectral imaging systems and software are used for quantitative analysis of biomarkers.
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