1：Experimental Design and Method Selection:

The method is based on the use of CdSe/SiO2 quantum dot microbeads (QBs) with a mean diameter of 106 nm. These have strong red luminescence (with excitation/emission peaks at 365/622 nm) which results in enhanced sensitivity. The QBs binding with monoclonal antibodies (mAbs) as the signal probes can react specifically with AFB1, ZEN and DON, respectively.

2：Sample Selection and Data Sources:

Naturally contaminated feedstuff including maize, wheat, distillers dried grains, peanut press pulp, shotcrete corn husk and corn germ meal samples were provided by National Research Center for Feed Engineering Technology.

3：List of Experimental Equipment and Materials:

CdSe quantum dot microbeads were purchased from Wuhan Jiayuan Quantum Dots Co., Ltd. The materials of the immunochromatographic strip including sample pad, absorbent pad, PVC pad and Nitrocellulose (NC) membrane were purchased from Millipore Corporation.

4：Experimental Procedures and Operational Workflow:

The QBs with a mean diameter of

5：3 nm were dissolved in deionized water at a concentration of 1 μM and stored in a 4 °C for further use. Three QBs labeled monoclonal antibodies (mAbs) were synthetized by conjugating QBs-COOH with anti-AFB1 mAbs, anti-ZEN mAbs and anti-DON mAbs. Data Analysis Methods:

1 The fluorescence signal intensities on the T1 (FlT1, AFB1-BSA), T2 (FlT2, ZEN-BSA), T3 (FlT3, DON-BSA) and C (FlC, the control) lines were measured by a fluorescent strip reader.