研究目的
Investigating the therapeutic and diagnostic applications of multifunctional magnetic nanoparticles (MFMNPs) for stem cell tracing and targeted drug delivery.
研究成果
The study demonstrates the potential of multifunctional magnetic nanoparticles for stem cell tracing and targeted drug delivery in cancer therapy. The FMNPs showed promising results in hyperthermia and bioimaging applications, suggesting their utility in theranostic approaches. Future studies should focus on optimizing the nanoparticles for clinical applications.
研究不足
The study is limited to in vitro and in vivo mouse models, and the translation of these findings to human applications requires further research. The specificity and efficiency of FMNPs in targeting and treating cancer cells in humans remain to be fully explored.
1：Experimental Design and Method Selection:
The study focuses on the synthesis and application of fluorescent magnetic nanoparticles (FMNPs) for labeling mesenchymal stem cells (MSCs) and their use in hyperthermia and bioimaging.
2：Sample Selection and Data Sources:
Mouse marrow MSCs are labeled with amino-modified FMNPs and injected into a mouse model with subcutaneous gastric cancer.
3：List of Experimental Equipment and Materials:
Includes ferric chloride, ferrous sulfate, sodium hydroxide, cadmium chloride, thioglycolic acid, and various cell culture reagents.
4：Experimental Procedures and Operational Workflow:
Detailed steps for the preparation of Fe3O4 nanoparticles, CdTe quantum dots, silica coating and functionalization of FMCNPs, primary culture and identification of mouse MSCs, and in vitro cell viability assay.
5：Data Analysis Methods:
Fluorescence imaging, MRI, immunofluorescence assay, Prussian blue staining, and ICP-MS analysis are used to evaluate the results.
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Inductively coupled plasma mass spectrometry system
ICP-MS
Thermo Elemental X7
Used in instrumentation
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Dulbecco’s modified Eagle’s medium
DMEM
Gibco
Used in the primary culture and identification of mouse mesenchymal stem cells
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Laser confocal scanning microscope
Leica TCS SP5
Leica Microsystems
Used in instrumentation
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Photoluminescence spectroscope
Perkin Elmer LS 55 spectrofluorometer
PerkinElmer
Used in instrumentation
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Superconducting quantum interference device magnetometer
PPMS-9 T
Quantum Design
Used in instrumentation
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Light microscope
Olympus IX71
Olympus
Used in instrumentation
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Transmission Electronic Microscope
JEOL JEM2010
JEOL Co. Ltd.
Used in instrumentation
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Multiskan microplate reader
Thermo MK3
Thermo Scientific
Used in instrumentation
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Cryostat
CM1900
Leica
Used in instrumentation
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Ferric chloride
FeCl3·6H2O
Used in the preparation of Fe3O4 nanoparticles
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Ferrous sulfate
FeSO4·7H2O
Used in the preparation of Fe3O4 nanoparticles
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Sodium hydroxide
NaOH
Used in the preparation of Fe3O4 nanoparticles and CdTe quantum dots
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Cadmium chloride
CdCl2·2.5H2O
Used in the preparation of CdTe quantum dots
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Thioglycolic acid
TGA
Used in the preparation of CdTe quantum dots
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Tellurium
Te
Used in the preparation of CdTe quantum dots
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Sodium borohydride
NaBH4
Used in the preparation of CdTe quantum dots
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Cyclohexane
C6H12
Used in silica coating and functionalization of FMCNPs
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T-octylphenoxypolyethoxyethanol
TritonX-100
Used in silica coating and functionalization of FMCNPs
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N-hexanol
C6H14O
Used in silica coating and functionalization of FMCNPs
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Tetraethyl orthosilicate
TEOS
Used in silica coating and functionalization of FMCNPs
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