研究目的
To determine whether different energy levels of CO2 fractional laser irradiation on skin in vitro could produce a non-ablative, micro-ablative, and ablative effect and to examine the features of the MTZs to better understand the features of the CO2 fractional laser and provide more reference bases for clinical treatment.
研究成果
For the first time, this study demonstrated that a CO2 fractional laser could achieve non-ablative, micro-ablative, and ablative effects on the skin after irradiation with different energy levels. These results provide new theoretical bases and observation measures for a better understanding of the features of ablative fractional lasers. These results also confirmed that under our parameter settings, there was additional capacity for adjustment of the laser energy level to ensure safe and effective treatment and reduce side effects.
研究不足
The study is an in vitro study, and further study is required to determine whether in vivo irradiation of human skin also has similar results. The pathological results were not quite consistent with those under DM and RCM, and both the width and depth values were larger than those in RCM.
1：Experimental Design and Method Selection:
In vitro irradiation of foreskin was performed using a CO2 fractional laser. Foreskin specimens were divided into 4 groups that received different amounts of irradiation energy, based on the number of irradiation passes they received: 5, 10, 15, and 20 passes, respectively. This corresponds to fluence energy of
2：3, 6, 9, 3 J/cmImmediately after irradiation, digital microscopy (DM), RCM, and histopathology were performed to observe whether the non-ablative, micro-ablative, and ablative phenomenon occurred, and the injury features of MTZs. Sample Selection and Data Sources:
A total of 8 samples of fresh foreskin tissues obtained after circumcision were selected as the study subjects and stored in a refrigerator. CO2 fractional laser irradiation was performed within 6 h of material collection. Each sample of foreskin was cut into 2–4 small specimens with a size of approximately 1 × 2 cm.
3：List of Experimental Equipment and Materials:
CO2 fractional laser (ZW-CL30, China Medical Laser (Beijing) Technology Co., Ltd.), digital microscopy (Anyty, 3R-MSUSB401, Japan, 3R, × 20 magnification), reflectance confocal microscopy (Vivascope 1500, Lucid, Incorporated, Rochester, NY, USA).
4：Experimental Procedures and Operational Workflow:
The parameters of the CO2 fractional laser included a 23-W output power, less than 1-ms pulse width, 1 cm × 1 cm scanning area, 600-μm emitted microbeam diameter, and a rectangular scanning mode. The distances of the rows and columns between each MTZ were both 1 mm; therefore, 81 MTZs could be scanned within 1 cm2, and the scanning density was 81 MTZs/cm
5：Data Analysis Methods:
The actual cross-sectional areas of MTZs were calculated for an oval morphology, and the mean value was obtained. In addition, the percentages of the actual injury areas in multiple MTZs were calculated. Finally, the statistical relationships between the measured injury area and the percentage of the injury area and the theoretical values for MTZs in the same location under different numbers of passes were compared using SPSS (IBM SPSS Statistics 21).
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