研究目的
Investigating the therapeutic effects of multifunctional single-layered graphene quantum dots (s-GQDs) on mitochondrial malfunction-related diseases.
研究成果
The novel s-GQDs with green fluorescence were successfully constructed and demonstrated strong binding affinity for ATP and good mitochondria targeting ability. They were effective in discriminating between SMCs from WT and SKI mice, indicating their potential as probes for studying cell metabolism and mitochondrial malfunction-related diseases. Targeting ER stress was shown to be an effective way to modulate metabolic pathways relevant to SERCA 2 inactivity directed mitochondrial dysfunction.
研究不足
The study focuses on the use of s-GQDs for monitoring ATP levels in a specific mouse model of mitochondrial dysfunction. The applicability to other models or human cells requires further investigation.
1：Experimental Design and Method Selection:
The s-GQDs were synthesized by condensing perylene tetracarboxylic anhydride (PTCDA) with polyethylenimine (PEI). The methodology included the synthesis of s-GQDs, their characterization, and application in monitoring ATP levels in cells.
2：Sample Selection and Data Sources:
Primary smooth muscle cells (SMCs) from wild-type (WT) and heterozygote Sarco/endoplasmic reticulum Ca2+ ATPase (SERCA) 2 C674S knock-in (SKI) mice were used.
3：List of Experimental Equipment and Materials:
UV-3600 spectrophotometer, F-7000 fluorescence spectrophotometer, high-resolution TEM, atomic force microscope, fluorescence microscope, laser confocal fluorescence microscopy.
4：Experimental Procedures and Operational Workflow:
The s-GQDs were synthesized, characterized, and then used to monitor ATP levels in SMCs from WT and SKI mice. The cells were treated with ER stress inhibitors (4-PBA, 4μ8c) to observe ATP fluctuation.
5：Data Analysis Methods:
Fluorescence intensity was measured to monitor ATP levels. Western blotting was used to analyze protein expression levels.
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