研究目的
Investigating the development of an antibody-free immunoassay for the specific determination of the carcinoembryonic antigen (CEA) in human serum using a boronate affinity molecularly imprinted polymer (MIP) and surface enhanced Raman scattering (SERS) tags.
研究成果
The study successfully demonstrated a SERS antibody-free immunoassay based on a boronate-affinity MIP array for rapid and sensitive detection of CEA from human serum samples. The method provides a new strategy for clinical glycoprotein detection but requires further research to improve simplicity, practicality, and signal-to-noise ratio.
研究不足
The synthesis of MIP arrays and AuNP requires considerable skill, and the method is limited in the detection of low concentration biological samples due to background noise in Raman spectroscopy.
1：Experimental Design and Method Selection:
The study utilized a boronate affinity molecularly imprinted polymer (MIP) and surface enhanced Raman scattering (SERS) tags for the detection of CEA.
2：Sample Selection and Data Sources:
Human serum samples from healthy individuals and liver cancer patients were used.
3：List of Experimental Equipment and Materials:
Included 4-vinylbenzeneboronic acid (VPBA), ethylene glycol dimethacrylate (EGDMA), gold nanoparticles, and 4-mercaptophenylboronic acid (MPBA).
4：Experimental Procedures and Operational Workflow:
Involved the synthesis of the boronate affinity array, specific capture of the glycoprotein, and formation of a sandwich structure for SERS detection.
5：Data Analysis Methods:
SERS measurements were performed to quantify CEA.
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