研究目的
To develop a controllably prepared core-shell plasmonic nanostructure coated with molecularly imprinted polymer (MIP) for sensitive and specific plasmon-enhanced fluorescence (PEF) assay, using riboflavin (RF) as a test compound.
研究成果
The study successfully developed a controllably prepared molecularly imprinted plasmonic nanostructure for sensitive and specific PEF assay. The hybrid nanostructure combined the high sensitivity of PEF detection with the high specificity of MIP, providing a promising nanomaterial for PEF assays. The method was successfully applied to the determination of RF in human urine, demonstrating its potential for real-world applications. The boronate affinity-based controllable oriented surface imprinting approach can be extended to a wide range of compounds, indicating broad applicability in the future.
研究不足
The study is limited to the use of RF as a test compound, and the applicability to other fluorophores needs further investigation. The linear range of the assay is relatively narrow, which may limit its use for samples with high RF concentrations. Additionally, the cross-reactivity with structurally similar compounds like FMN, VB12, and VB9 could affect the assay's specificity in complex samples.
1：Experimental Design and Method Selection:
The study involved the synthesis of RF-imprinted Ag@SiO2 nanoparticles in a controllable manner to provide an optimal distance between the metal surface and RF molecules. The methodology included the use of boronate affinity-based oriented surface imprinting approaches for controllable nanoscale fabrication of MIPs.
2：Sample Selection and Data Sources:
RF was used as a test compound. The study also involved the use of human urine samples for method validation.
3：List of Experimental Equipment and Materials:
Instruments included TEM, SEM, DLS, BET and BJH analyzers, fluorescence spectrometer, UV-vis spectrophotometer, and SERS microscope. Materials included AgNO3, trisodium citrate, TEOS, APTES, RF, and other chemicals for synthesis and characterization.
4：Experimental Procedures and Operational Workflow:
The procedure included the synthesis of AgNPs, silica NPs, Ag@SiO2 hybrid nanostructure, and RF-imprinted Ag@SiO2 NPs. The thickness of the silica shell and imprinting layer was controlled by adjusting the reaction time. The binding isotherms, imprinting effect, and PEF enhancement were characterized.
5：Data Analysis Methods:
Data analysis involved the use of FDTD simulations for electric field enhancement, Scatchard equation for binding analysis, and fluorescence intensity measurements for PEF assay.
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