研究目的
To investigate the fabrication and characterization of novel cRGD modified graphene quantum dots for chemo-photothermal combination therapy, focusing on drug loading capacity, photothermal efficiency, cellular uptake, and combination therapeutic effect.
研究成果
The R–GQDs@DOX system demonstrated significant potential for chemo-photothermal combination therapy, with high drug loading capacity, pH-responsive drug release, and effective cellular uptake. The combination therapy showed enhanced inhibition of tumor cell growth compared to individual therapies.
研究不足
The study focuses on in vitro experiments, and further in vivo studies are needed to validate the therapeutic efficacy and safety of R–GQDs@DOX. The targeting efficiency and potential side effects in a physiological environment were not fully explored.
1：Experimental Design and Method Selection:
The study involved the synthesis of cRGD-modified graphene quantum dots (R–GQDs) and their loading with doxorubicin (DOX) to form R–GQDs@DOX. The structure was characterized using FT-IR spectrum, UV–vis spectrum, and AFM.
2：Sample Selection and Data Sources:
H460 and SK–mel–5 cell lines were used as model cells for cytotoxicity and imaging tests.
3：List of Experimental Equipment and Materials:
Included PEGylated N-succinimidyl 3-(2-pyridyldithio)-propionate, graphene quantum dots, DOX, and c[RGDfK (Mpa)].
4：Experimental Procedures and Operational Workflow:
The synthesis involved coupling GQDs with cRGD using a thiolation reaction, followed by loading with DOX. The drug loading efficiency was calculated, and the photothermal effect was tested under near-infrared light.
5：Data Analysis Methods:
The drug release behavior was determined using the dynamic dialysis method, and cytotoxicity was assessed using the CCK-8 method.
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