研究目的
Developing a fast, sensitive and highly selective method for simultaneous detection of multiple heavy metal ions (Hg2+, Pb2+, and Ag+).
研究成果
The developed fluorescence quantitative analysis method allows for the simultaneous detection of Hg2+, Pb2+, and Ag+ with high accuracy, selectivity, and low detection limits. It is applicable to real soil samples and offers a fast detection speed.
研究不足
The method requires specific conditions for optimal performance, including precise pH, temperature, and ionic strength. The presence of other metal ions at high concentrations could potentially interfere with the detection, although the method shows good selectivity.
1：Experimental Design and Method Selection:
Designed three nucleic acid aptamer probes (PHg, PPb, PAg) with different fluorophores (FAM, TAMRA, Cy-5) and used graphene oxide (GO) as a quencher. The method is based on the fluorescence resonance energy transfer (FRET) between the fluorophores and GO.
2：Sample Selection and Data Sources:
Soil samples were taken from Enshi, Hubei Province, China, and prepared for analysis.
3：List of Experimental Equipment and Materials:
RF-5301PC fluorescence spectrophotometer, PHS-3C potentiometer, graphene oxide, aptamer probes synthesized by Sangon Biotechnology Co., Ltd.
4：Experimental Procedures and Operational Workflow:
Aptamer probes were mixed with metal ions, incubated, and then GO was added. Fluorescence measurements were performed to monitor the reaction process.
5：Data Analysis Methods:
The fluorescence signals of FAM, TAMRA, and Cy-5 were measured using synchronous scanning fluorescence spectroscopy to determine the concentration of metal ions.
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