研究目的
To develop a novel “turn off-on” sensor for detection of interaction between DNA and acrylamide (ACR) using L-cysteine capped Mn-doped ZnS quantum dots (QDs) as room temperature phosphorescent probes.
研究成果
The developed biosensor is simple, selective, and does not require any derivatization step or sample pretreatment. It provides a novel method for evaluating the interaction between DNA and ACR, with potential applications in understanding the effects of carcinogenic molecules on DNA.
研究不足
The study focused on the interaction between DNA and ACR using a specific type of QDs. The applicability of the method to other types of interactions or QDs was not explored.
1：Experimental Design and Method Selection:
The study utilized L-cysteine capped Mn-doped ZnS QDs as phosphorescent probes to detect the interaction between DNA and ACR. The method was based on the quenching and recovery of the phosphorescence signal of QDs.
2：Sample Selection and Data Sources:
Calf thymus double-stranded DNA and ACR were used. The DNA solution was prepared by dissolving DNA in deionized water, and its purity was confirmed spectrophotometrically.
3：List of Experimental Equipment and Materials:
A Cary Eclipse fluorescence spectrophotometer for phosphorescence measurements, a Specord 50 Plus for UV-Vis spectra, and an Orion model 720 A pH meter for pH measurements were used.
4：Experimental Procedures and Operational Workflow:
The interaction was studied by adding ACR to QDs solution, observing the quenching effect, and then adding DNA to observe the recovery of phosphorescence intensity. UV-Vis spectrophotometry was also used to study the interaction.
5：Data Analysis Methods:
The quenching mechanism was analyzed using Stern–Volmer and Lineweaver–Burk equations. The binding constant and number of binding sites were calculated.
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