研究目的
To develop a low-cost, selective, and sensitive analytical procedure for assessing total mercury in fishery products using room temperature phosphorescence quenching of a phenobarbital-containing polymer/silica coated Mn-doped ZnS quantum dots composite.
研究成果
The developed RTP-based Ph-QDs method is a highly selective, sensitive, reliable, and cost-effective option for quality control of total mercury in fishery products. It offers comparable results to more sophisticated analytical techniques like ICP-MS but requires low-cost instrumentation.
研究不足
The method requires a stabilization time of 12 min for Hg (II)-Ph-QDs interaction. The use of Ph-QDs at concentrations higher than 20 mg L?1 led to a weak RTP quenching effect.
1：Experimental Design and Method Selection
The methodology involves the synthesis of a phenobarbital-containing polymer/silica coated Mn-doped ZnS quantum dots composite (Ph-QDs) for the selective recognition of mercury species in fish samples. The RTP quenching effect of the composite is utilized for mercury detection.
2：Sample Selection and Data Sources
Fish samples including yellowfin tuna, swordfish, bluefin tuna, and blue shark were purchased from local fish markets. A certified reference material (BCR-463, tuna fish) was also analyzed for method validation.
3：List of Experimental Equipment and Materials
Fluorescence/phosphorescence spectrophotometer, TEM-EDS, XRD, FTIR, ICP-MS, microwave accelerated system, pH meter, ultrasonic cleaner bath, ultracentrifuge, blade grinder, analytical balance.
4：Experimental Procedures and Operational Workflow
The synthesis of Ph-QDs, preparation of fish samples, ultrasound-assisted acid extraction, microwave-assisted acid digestion, RTP measurements, and ICP-MS measurements were conducted under optimized conditions.
5：Data Analysis Methods
The RTP quenching was calculated according to the Stern–Volmer equation. The sensitivity of the method was estimated by calculating the limit of detection (LOD) and limit of quantification (LOQ). Precision and accuracy were evaluated at different Hg (II) concentration levels.
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