研究目的
To improve the photoluminescence efficiency of upconversion nanoparticles (UCNPs) and their application in photodynamic therapy (PDT) by using semiconductor quantum dots (QDs) as sensitizers.
研究成果
The study demonstrates that Ag2Se QDs can significantly enhance the photoluminescence efficiency of UCNPs through a resonance energy transfer process, leading to improved therapeutic performance in PDT. This approach overcomes the limitations of UCNPs and opens new avenues for their application in bioimaging and therapy.
研究不足
The study focuses on Ag2Se QDs and Nd3+/Yb3+ co-doped UCNPs, and the findings may not be directly applicable to other types of QDs or UCNPs. The in vivo studies were limited to specific tumor models.
1：Experimental Design and Method Selection:
The study involved the synthesis of Ag2Se QDs and Nd3+/Yb3+ co-doped UCNPs, followed by their co-encapsulation into amphiphilic phosphatidylcholine (PC) to form UCNPs-QDs composites. The energy transfer from QDs to Yb3+ was confirmed through luminescence enhancement and lifetime studies.
2：Sample Selection and Data Sources:
HeLa and HCT116 cells were used for in vitro studies, and Lewis lung carcinoma (LLC) and 4T1 tumor-bearing mice were used for in vivo studies.
3：List of Experimental Equipment and Materials:
Transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared (FTIR) spectra, confocal laser scanning microscopy (CLSM), and flow cytometry were used.
4：Experimental Procedures and Operational Workflow:
The synthesis of UCNPs and QDs, their encapsulation, and application in PDT were detailed.
5：Data Analysis Methods:
The UCL enhancement factor, intracellular 1O2 generation, and therapeutic performance were analyzed.
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