摘要： Efficient separation of electron-hole pairs is vitally crucial to enhancing the analytical performance of paper-based photoelectrochemical (PEC) bioanalysis. Herein, a simple but effective strategy is developed to modulate the effective separation of photogenerated electrons and holes via introducing a polar charge carriers-created (PCC) electric field induced by a classical perovskite ferroelectric BaTiO3 (BTO). By inserting it between the n-type WO3 nanoflakes and p-type Cu2O (WO3 nanoflakes/BTO/Cu2O), the photoelectrode is endowed with a renewable PCC electric field, as a sustaining driving force, to guarantee the realization of directional separation of charge carrier (DSCC) strategy in PEC bioanalysis. The enduring PCC electric field can attract the electrons of Cu2O and holes of WO3, respectively, thereby regulating the directional migration of charge carriers and achieving an enhanced PEC photocurrent for the ultrasensitive quantification based on the highly efficient separation of electron-hole pairs. Consequently, with respect to WO3 nanoflakes/Cu2O and WO3 nanoflakes photoelectrode, the polarized WO3 nanoflakes/BTO/Cu2O photoelectrode exhibits 1.7 and 10.9 times higher photocurrent density, respectively. Benefiting from this, the prominent photocurrent density is obtained which is extremely beneficial for enhancing the sensitivity of PEC bioanalysis. Ultimately, the ultrasensitive detection of model prostate specific antigen (PSA) is realized and presented a linear range of 0.1 pg/mL-50 ng/mL with the detection limitation of 0.036 pg/mL. This work provides the basis for understanding the role of the polarized electric field induced by ferroelectric in tuning the charge separation as well as insights on strategies for constructing high-performance paper-based PEC bioanalysis.