研究目的
To design a nanoscale radiosensitizer by grafting 2-deoxy-d-glucose (2DG) on to graphene quantum dots (GQD) to achieve osteosarcoma (OS) targeting and boost radiotherapy (RT) efficacy.
研究成果
The use of 2DG-g-GQDs as an OS-targeting radiosensitizer improves the therapeutic outcome and exhibits potential for use in low-dose precision RT for OS. The combination of 2DG-g-GQDs and RT significantly enhanced oxidative stress and DNA damage in OS cells, inhibited tumour progression and lung metastasis in a mouse model.
研究不足
The study is limited by the radioresistant nature of OS, which may require high radiation doses. The potential systemic toxicity of conventional radiosensitizers was not compared directly with the nanoparticle-based approach in this study.
1：Experimental Design and Method Selection:
The study involved the synthesis of 2DG-grafted GQDs (2DG-g-GQDs) and their characterization using FTIR, Raman spectroscopy, and TEM. The radiosensitizing effect was evaluated in vitro and in vivo using the 143B OS cell line and an OS xenograft mouse model.
2：Sample Selection and Data Sources:
The human OS cell line (143B) and a normal human osteoblast cell line (hFOB 1.19) were used. In vivo studies were conducted on nude mice with OS xenografts.
3：19) were used. In vivo studies were conducted on nude mice with OS xenografts.
List of Experimental Equipment and Materials:
3. List of Experimental Equipment and Materials: Equipment included a transmission electron microscope (JEM-2000EX II, JEOL), UV-vis spectrometer (V-630 Spectrophotometer, JASCO), and confocal microscope. Materials included graphite powder, sodium nitrate, concentrated H2SO4, KMnO4, and 2-deoxy-d-glucose.
4：Experimental Procedures and Operational Workflow:
The synthesis of GQDs and 2DG-g-GQDs, cytotoxicity assays, cellular uptake studies, clonogenic survival assays, ROS detection, DNA double-strand break monitoring, and in vivo therapeutic efficacy studies were conducted.
5：Data Analysis Methods:
Data were analyzed using ImageJ software for quantification of cellular uptake, colony formation, and ROS production. Statistical analysis was performed using ANOVA and multiple comparisons.
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