1：Experimental Design and Method Selection:

The study utilized a thermoplasmonic device consisting of a one-dimensional metal grating for efficient light absorption and heat generation. A thin-film thermocouple was integrated for temperature monitoring. The device was controlled by an Arduino microcontroller for automated PCR assays.

2：Sample Selection and Data Sources:

Genomic DNAs were extracted from Acinetobacter baumannii, Klebsiella pneumonia, Escherichia coli, and Campylobacter for PCR amplification.

3：List of Experimental Equipment and Materials:

The setup included an 808 nm laser, the thermoplasmonic device, a PCR reaction chamber, a fluorescence detector, and an Arduino microcontroller. Materials included a silicone chamber, transparent plastic film, and acrylic slides.

4：Experimental Procedures and Operational Workflow:

The device was illuminated by a near-infrared laser to heat the PCR solution. Temperature was monitored and controlled via the integrated thermocouple. PCR assays included multiplex PCR and qPCR for gene amplification and analysis.

5：Data Analysis Methods:

Fluorescence intensity was measured for qPCR analysis. Gel electrophoresis was used to analyze PCR products.