研究目的
To develop a sensitive and label-free electrochemiluminescence (ECL) immunosensor for the detection of aflatoxin B1 (AFB1) in complex matrices like lotus seeds.
研究成果
The developed ZnCdS@ZnS QDs–based label-free ECL immunosensor demonstrated high sensitivity, selectivity, stability, and reproducibility for the detection of AFB1 in lotus seed samples. It offers a practical platform for trace detection of mycotoxins in complex matrices, with potential applications in food safety, environmental monitoring, and disease diagnostics.
研究不足
The study does not mention the potential interference from other mycotoxins or complex matrix components in real samples beyond the tested selectivity against OTA and ZEA. The stability and reproducibility were tested under controlled conditions, and real-world application might present additional challenges.
1：Experimental Design and Method Selection:
The study involved the design of a label-free ECL immunosensor using ZnCdS@ZnS QDs as signal probes and Nafion for assembling QDs on the Au electrode surface. Anti-AFB1 antibodies were used as capturing elements.
2：Sample Selection and Data Sources:
Lotus seed samples were prepared by crushing, sifting, and extracting with methanol solution.
3：List of Experimental Equipment and Materials:
ZnCdS@ZnS QDs, Nafion solution, Au electrode, anti-AFB1 antibodies, AFB1 standard solution, and various chemicals for buffer preparation.
4：Experimental Procedures and Operational Workflow:
The Au electrode was polished, modified with QDs and Nafion, conjugated with anti-AFB1 antibodies, and then incubated with AFB1 samples. ECL measurements were performed in a three-electrode system with K2S2O8 as a co-reactant.
5：Data Analysis Methods:
The ECL signals were recorded and analyzed to quantify AFB1 concentrations based on the decrease in ECL intensity.
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