研究目的
To develop a novel substrate for surface-enhanced laser desorption/ionization mass spectrometry (SELDI-MS) using a spherical vinyl-functionalized covalent-organic framework (COF-V) for the sensitive detection of small molecules and glucose in diabetic urine.
研究成果
The spherical COF-V substrate demonstrated superior performance in SELDI-MS for the analysis of small molecules and glucose in diabetic urine, offering high sensitivity, no matrix background interference, and excellent salt tolerance. This work expands the application of COFs in mass spectrometry and provides a promising tool for clinical diagnosis.
研究不足
The study primarily focuses on the application of spherical COF-V in SELDI-MS for small molecule analysis and glucose detection in diabetic urine. The generalizability to other types of molecules or clinical samples may require further investigation.
1：Experimental Design and Method Selection:
The study involved the synthesis of spherical COF-V at room temperature and its application as a substrate in SELDI-MS for the analysis of small molecules and glucose in diabetic urine.
2：Sample Selection and Data Sources:
Various biological micromolecules and environmental pollutants were selected as model compounds. Diabetic and normal urine samples were collected for glucose analysis.
3：List of Experimental Equipment and Materials:
A Bruker Autoflex Ⅱ mass spectrometer was used for mass spectrometry analysis. Materials included 1,3,5-tris(4-aminophenyl) benzene (Tab), 2,5-Divinylterephthalaldehyde (Dva), and other chemicals for COF-V synthesis and analysis.
4：Experimental Procedures and Operational Workflow:
The spherical COF-V was synthesized, characterized, and then used as a substrate in SELDI-MS for the analysis of selected molecules and glucose in urine samples.
5：Data Analysis Methods:
Mass spectra were analyzed to identify and quantify the molecules of interest, with a focus on glucose in diabetic urine.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
