研究目的
To develop a tunable biosensor for virus detection using localized surface plasmon resonance (LSPR) by controlling the distance between quantum dots (QDs) and gold nanoparticles (AuNPs) with a peptide chain.
研究成果
The developed biosensor demonstrates high sensitivity and specificity for influenza virus detection, with a wide linear range and low detection limit. The tunable distance between QDs and AuNPs via peptide linkage allows for optimized LSPR effects, making this biosensor a promising tool for virus surveillance.
研究不足
The study mentions the potential interference from thiolated compounds like cysteine, which could affect the sensor's selectivity. Additionally, the performance of the sensor degrades after three weeks, possibly due to antibody instability.
1：Experimental Design and Method Selection:
The study involved the synthesis of CdZnSeS/ZnSeS QDs and AuNPs, and their conjugation via a peptide chain to form a nanocomposite. The distance between QDs and AuNPs was controlled by varying the length of the peptide chain.
2：Sample Selection and Data Sources:
Influenza virus was used as the target analyte. The detection mechanism was based on the LSPR-induced fluorescence enhancement of QDs and its quenching upon virus binding.
3：List of Experimental Equipment and Materials:
Transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), dynamic light scattering (DLS), fluorescence emission and UV-Vis absorption measurements were used for characterization.
4：Experimental Procedures and Operational Workflow:
The nanocomposite was synthesized by conjugating QDs and AuNPs with a peptide chain, followed by antibody conjugation for virus detection. The fluorescence intensity was measured before and after virus addition.
5：Data Analysis Methods:
The change in fluorescence intensity was analyzed to determine the virus concentration, with a linear range of 10?14 to 10?9 g?mL?1 and a detection limit of 17.02 fg?mL?1.
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