研究目的
To develop a sensitive, low-cost biosensor based on laser induced graphene for femtomolar microRNA (miRNA) detection.
研究成果
The laser induced N-doped porous graphene biosensor demonstrates high sensitivity and specificity for miRNA detection, with a detection limit as low as 10 fM. The method offers a simple, cost-effective approach for miRNA detection, showing great potential for biomedical applications.
研究不足
The study focuses on a specific miRNA (hsa-miR-486-5p) and the detection method's applicability to other miRNAs or nucleic acids requires further investigation. The scalability and reproducibility of the LIG fabrication process in industrial settings need to be explored.
1：Experimental Design and Method Selection:
The laser induced graphene (LIG) was prepared by direct laser writing on commercial polyimide (PI) and patterned via a computer-aided design system as an electrode for electrochemical biosensing.
2：Sample Selection and Data Sources:
Preeclampsia specific miRNA hsa-miR-486-5p was magnetically purified and directly adsorbed on the surface of graphene electrode.
3：List of Experimental Equipment and Materials:
A continuous wave (CW) CO2 laser cutter system, DUPONT Kapton HN Film Tape, Potassium ferrocyanide, Potassium ferricyanide, phosphate buffer saline (PBS) tablets.
4：Experimental Procedures and Operational Workflow:
The LIG was fabricated with optimized laser power and scan speed. The target miRNAs were purified and adsorbed onto the LIG electrode for electrochemical detection.
5：Data Analysis Methods:
Differential pulse voltammetry (DPV) in the presence of [Fe(CN)6]3-/4- redox system was monitored to detect the amount of absorbed miRNA.
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