研究目的
To design and evaluate a novel microfluidic nanoplatform which successfully combines the efficiency of AuBPs as signal transducers with the sample-driven advantages of the microfluidic channels into a miniaturized, portable, low-cost, and sensitive dual-modal LSPR/SERS lab-on-a-chip device.
研究成果
The miniaturized portable microfluidic system successfully detects and identifies analyte molecules in laminal flow with high sensitivity, proving its potential for further complex biosensing applications.
研究不足
The study focuses on the proof-of-concept with p-ATP as the target molecule. Further complex biosensing applications would require validation with a broader range of analytes and under varying conditions.
1：Experimental Design and Method Selection:
The strategy involves two steps: i) the controlled deposition of gold bipyramidal nanoparticles (AuBPs) onto a functionalized solid glass substrate and ii) the integration of the as-fabricated plasmonic substrate into a polydimethylsiloxane (PDMS) microfluidic circuit.
2：Sample Selection and Data Sources:
The p-aminothiophenol (p-ATP) target molecule was used for LSPR-SERS validation.
3：List of Experimental Equipment and Materials:
Includes gold nanobipyramids, PDMS for microfluidic channel fabrication, and a syringe pump for analyte injection.
4：Experimental Procedures and Operational Workflow:
The microfluidic channel and the previously fabricated plasmonic substrate were plasma treated and bonded. The LSPR-SERS validation was performed by injecting the p-ATP solution into the microfluidic channel.
5：Data Analysis Methods:
The optical responses were monitored at refractive index changes, and the SERS detection performance was evaluated by monitoring the intensity of the Raman band located at 1585 cm-1.
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