研究目的
To develop a novel coumarin-based fluorescence chemosensor for the highly selective detection of Al3+ over other metal ions, with potential applications in biological systems.
研究成果
The sensor CND demonstrates excellent selectivity and sensitivity towards Al3+, with a low detection limit and high association constant. It is capable of recognizing intracellular Al3+ in live cells, making it a promising candidate for biological applications.
研究不足
The study focuses on the detection of Al3+ in a specific solvent mixture (EtOH-HEPES) and may require further validation in purely aqueous or other biological media for broader applicability.
1：Experimental Design and Method Selection:
The sensor CND was synthesized by condensation of 8-formyl-7-hydroxycoumarin with niacin hydrazide. The spectroscopic properties and binding mechanism with Al3+ were investigated.
2：Sample Selection and Data Sources:
Stock solutions of metal ions and the sensor CND were prepared in deionized water and DMSO, respectively.
3：List of Experimental Equipment and Materials:
UV-Vis spectrophotometer, fluorescence spectrometer, Bruker 400MHz NMR instrument, Thermo Fischer Nicolet IS50 FT-IR spectrometer, Bruker Thermo Scientific Q Exactive mass spectrometer, Dual photon confocal microscope.
4：Experimental Procedures and Operational Workflow:
Fluorescence responses of CND to various metal ions were measured. Fluorescence titration with Al3+ was performed to determine detection limits and association constants.
5：Data Analysis Methods:
The association constants were calculated using the Benesi-Hildebrand method. Fluorescence quantum yields were measured using quinine sulfate as the standard.
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