研究目的
Developing a sensitive fluorescence spectroscopy method for the determination of aminophylline (AP) using amino-functionalized graphene quantum dots (afGQDs) and investigating the fluorescence quenching mechanism induced by riboflavin (Rf) and the Rf-AP system.
研究成果
The study successfully developed a sensitive fluorescence spectroscopy method for the determination of AP using afGQDs. The fluorescence quenching mechanism was elucidated, involving dynamic quenching, inner filter effect, and photo-induced electron transfer. The method demonstrated a wide linear range and low detection limit, with successful application in pharmaceutical sample analysis.
研究不足
The study is limited to the analysis of AP in a controlled laboratory environment. The practical application in complex biological samples may require further validation. The sensitivity and selectivity of the method may be affected by the presence of other interfering substances.
1：Experimental Design and Method Selection:
The study involved the synthesis of afGQDs via a two-step method and their characterization using TEM, UV–vis absorption spectrum, and infrared spectrum. The fluorescence quenching mechanism was investigated by analyzing the fluorescence spectra of afGQDs in the presence of Rf, AP, and their mixture.
2：Sample Selection and Data Sources:
The samples included afGQDs, Rf, AP, and their mixtures in phosphate buffer saline (PBS) at pH 7.
3：List of Experimental Equipment and Materials:
5.
3. List of Experimental Equipment and Materials: Materials included citric acid, aqueous ammonia, Rf, AP, and other reagents. Equipment included an F-7000 spectrofluorimeter, Tecnai G2 20ST TEM, UV–2450 spectrophotometer, and Nicolet Nexus 670 FTIR spectrometer.
4：Experimental Procedures and Operational Workflow:
The synthesis of afGQDs, preparation of samples, and fluorescence measurements were conducted under controlled conditions. The fluorescence intensity changes were recorded under different pH values and Rf concentrations.
5：Data Analysis Methods:
The fluorescence intensity changes were analyzed using the Stern–Volmer equation to determine the quenching constant. The linear dependency between fluorescence intensity change and AP concentration was established.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
