研究目的
To propose and demonstrate a label-free thrombin detection method using a tapered fiber-optic interferometric aptasensor with high sensitivity and specificity.
研究成果
The tapered fiber-optic interferometric aptasensor demonstrates high sensitivity and specificity for label-free thrombin detection, with a lowest detectable concentration of 0.1 μM. The sensor's compact size and flexibility, combined with its performance, make it a promising tool for biomarker detection in biological and clinical applications.
研究不足
The study does not address potential interference from other biomolecules in complex biological samples beyond the demonstrated specificity tests. The temperature sensitivity of the sensor, although characterized, could affect measurements in non-controlled environments.
1：Experimental Design and Method Selection:
The study involves the fabrication of a tapered fiber-optic interferometric sensor for label-free thrombin detection. The sensor is based on refractive index (RI) measurement, with sensitivity determined by tracing the wavelength shift of a selected resonance in the transmission spectrum versus RI change.
2：Sample Selection and Data Sources:
The sensor is functionalized with thrombin-binding aptamers for specific thrombin detection. Experiments involve soaking the sensor in aptamer solutions of varying concentrations and measuring the response to thrombin in pure and mixed solutions.
3：List of Experimental Equipment and Materials:
A broadband source (BBS) and an optical spectrum analyzer (OSA) are used for light emission and spectrum recording, respectively. Chemicals include 3-Aminopropyl-triethoxysilane (APTES), glutaraldehyde, and Human α-thrombin protein sample.
4：Experimental Procedures and Operational Workflow:
The sensor is fabricated by tapering a commercial double-cladding fiber. Surface functionalization involves cleaning, silanization, crosslinking, and aptamer immobilization. Thrombin detection is performed by monitoring wavelength shifts in the transmission spectrum.
5：Data Analysis Methods:
The sensitivity is calculated based on wavelength shifts in response to RI changes. Specificity is assessed by comparing responses to thrombin and non-specific solutions like pepsin.
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