研究目的
Investigating the use of negative dielectrophoresis (DEP) spectroscopy as a transduction mechanism for the detection of the pancreatic cancer biomarker CA 19-9.
研究成果
Negative DEP spectroscopy is an effective label-free transduction mechanism for detecting cutoff levels of the pancreatic cancer biomarker CA 19-9, with potential applications in early-stage diagnosis and prognosis of pancreatic cancer.
研究不足
The study was conducted in PBS buffer, and future work is needed to extend this method to spiked serum samples and human samples. Additionally, the current method requires expert technician alignment for image processing.
1：Experimental Design and Method Selection:
The study utilized negative DEP spectroscopy to measure the concentration of the pancreatic cancer biomarker CA 19-
2：The method involved the use of functionalized polystyrene microspheres (PM) and a pearl-shaped interdigitated gold micro-electrode to apply an electric field. Sample Selection and Data Sources:
Biotinylated PM with 750 nm diameter were functionalized with Streptavidin and biotinylated CA 19-9 antibody, then exposed to different concentrations of CA 19-9 antigen.
3：List of Experimental Equipment and Materials:
Equipment included a pearl-shaped interdigitated electrode, OMFL600 low power microscope, Tektronix AFG series function generator, and a custom-built green LED lamp. Materials included biotinylated PM, Streptavidin, biotinylated CA 19-9 antibody, and CA 19-9 antigen.
4：Experimental Procedures and Operational Workflow:
The experiment involved applying positive DEP to attract PM to the electrode edges, then switching to negative DEP to repel PM, with the velocity of repulsion measured using real-time image processing.
5：Data Analysis Methods:
The drift velocity of PM due to negative DEP was calculated from images captured at different frequencies, with the center of mass of the PM layer used to determine the speed of repulsion.
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