研究目的
To develop and simplify label-free fiber optic biosensors based on immobilization of oligonucleotides on dual-peak long period gratings (dLPGs) for disease analysis and medical diagnosis.
研究成果
The study successfully demonstrated a label-free biosensor based on a dual-peak LPG for detecting oligonucleotide hybridization in real-time. The one-step EDC-mediated procedure facilitated the covalent immobilization of 5′-phosphorylated oligonucleotide on an amino-modified glass fiber sensor surface, showing high sensitivity and specificity. This technology has potential for future aptamer-based binding studies and disease analysis.
研究不足
The study focuses on the immobilization of oligonucleotides and their hybridization, with potential limitations in the detection of other biomolecules or in more complex biological matrices.
1：Experimental Design and Method Selection:
The study employed a one-step EDC-mediated reaction for the immobilization of unmodified oligonucleotides on the glass fiber surface along the grating region.
2：Sample Selection and Data Sources:
A 5'-phosphorylated oligonucleotide was used for immobilization, and its hybridization with complementary oligonucleotides was monitored.
3：List of Experimental Equipment and Materials:
Materials included APTES, EDC, 1-methylimidazole, PBS, SSPE buffer, HCl, DI water, and methanol. Equipment included a CW frequency-doubled Ar laser for grating fabrication and an optical spectrum analyzer for monitoring spectral changes.
4：Experimental Procedures and Operational Workflow:
The process involved cleaning the glass fiber, silanization with APTES, oligonucleotide immobilization via EDC-mediated reaction, and hybridization monitoring.
5：Data Analysis Methods:
Spectral evolution was monitored using an optical spectrum analyzer, and data analysis was performed using a customized program for defining resonance wavelengths.
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