研究目的
Exploring more precise cell membrane structures and detailed functions of biomolecules on cell membranes using high resolution microscopy techniques like AFM and NSOM.
研究成果
AFM-SMFS, AFM-TREC, and NSOM are powerful techniques for in-situ single molecule detection of biomolecule distributions on cell membranes, providing insights into the roles and mechanisms of membrane biomolecules in biological phenomena.
研究不足
AFM and NSOM are surface-limited detection techniques, unable to image intracellular molecules. AFM's temporal resolution is limited for dynamic studies of cell membrane biomolecules. NSOM requires fluorescent markers for biomolecule detection.
1：Experimental Design and Method Selection:
The study utilizes AFM and NSOM for high-resolution imaging of cell membrane biomolecules. AFM is used for single molecule detection by measuring cantilever deflection, while NSOM combines high resolution morphology and fluorescence images.
2：Sample Selection and Data Sources:
The study focuses on cell membranes of bacterial and mammalian cells, using specific ligands and fluorescence markers for biomolecule detection.
3：List of Experimental Equipment and Materials:
AFM tips functionalized with specific ligands, NSOM probes with small apertures, and fluorescence markers.
4：Experimental Procedures and Operational Workflow:
Functionalization of AFM tips with ligands, scanning of cell surfaces with AFM and NSOM, and data collection on biomolecule distribution and interactions.
5：Data Analysis Methods:
Analysis of force-distance curves for AFM and fluorescence signals for NSOM to map biomolecule distribution and interactions.
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