研究目的
To establish a fast and accurate ultraviolet-visible (UV-vis) spectrophotometric method for determining the presence of chlorogenic acid (CGA) in fermentation broth and fruits.
研究成果
The UV-vis spectrophotometric method is accurate, rapid, sensitive, and cost-effective for quantifying CGA in biological samples, showing good correlation with HPLC results.
研究不足
The method's accuracy may be affected by the presence of polysaccharides in the fermentation broth. The study suggests further optimization for samples with complex matrices.
1：Experimental Design and Method Selection:
A UV-vis spectrophotometric method was established using a potassium ferricyanide-Fe (III) detection system. The method was compared with HPLC for validation.
2：Sample Selection and Data Sources:
CGA was determined in fermentation broth and mango fruits. Standard CGA solutions were prepared for calibration.
3：List of Experimental Equipment and Materials:
Epoch 7200200 micro-plate spectrophotometer, Waters HPLC system, JADE-PAK ODS-AQ C18 column, and various chemicals including potassium ferricyanide, ferric chloride, and solvents like ethanol, methanol, and DMSO.
4：Experimental Procedures and Operational Workflow:
The reaction conditions (temperature, pH, reaction time) were optimized. The absorbance was measured at 790 nm. HPLC analysis was performed for comparison.
5：Data Analysis Methods:
The linearity, precision, accuracy, and recovery of the method were assessed. Statistical analysis was performed using Origin 8.5.
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