1：Experimental Design and Method Selection:

The study involved the synthesis of amphiphilic star or multi-arm block copolymers through the Atom Transfer Radical Polymerization (ATRP) method using hydrophilic 4-arm polyethylene glycol (4-arm-PEG) as an initiator. The structure-property relationship was investigated among five block copolymers, with and without fluorine-containing moieties.

2：Sample Selection and Data Sources:

The study used hydrophobic oxygen probe PtTFPP, Escherichia coli (E. coli) JM109, macrophage cell J774A.1, and tumor-bearing mice for in vivo hypoxia imaging.

3：4A.1, and tumor-bearing mice for in vivo hypoxia imaging. List of Experimental Equipment and Materials:

3. List of Experimental Equipment and Materials: Materials included PtTFPP, hexafluorobutyl methacrylate (HFMA), styrene, tri?uoroethyl methacrylate (TFEMA), 4-arm-PEG-OH, and others. Instruments included Water 1515 gel permeation chromatography (GPC), nuclear magnetic resonance spectroscopy (NMR), dynamic light scattering (DLS), fluorescence spectrofluorophotometer, and a plate reader.

4：Experimental Procedures and Operational Workflow:

The synthesis of block copolymers, preparation of micelles with PtTFPP, determination of critical micelle concentrations, quantum efficiency determination, response to oxygen, response time measurement, phosphorescence lifetime determination, E. coli culture, cell culture, and in vivo imaging were performed.

5：Data Analysis Methods:

The study analyzed the photophysical properties of the micelles, oxygen sensing properties, and the application of the sensors for monitoring cellular respiration and in vivo hypoxia imaging.