研究目的
To develop a rapid, cost-effective, portable, and user-friendly method for the in-situ detection of live, dead, and antibiotic-resistant bacteria using silver nanorods array.
研究成果
The study successfully demonstrated a rapid, cost-effective, and portable method for detecting live, dead, and antibiotic-resistant bacteria using AgNRs arrays. The method is based on the detection of H2S gas emitted by live bacteria, with results obtainable within 6 hours, significantly faster than conventional methods. The developed mobile app 'Colorimetric Detector' enhances the user-friendliness and field deployability of the technique.
研究不足
The method relies on the production of H2S by live bacteria, which may vary among different bacterial species. The sensitivity and specificity of the AgNRs array to H2S need further validation across a wider range of bacterial species and environmental conditions.
1:Experimental Design and Method Selection:
The study utilized silver nanorods (AgNRs) arrays fabricated by the glancing angle deposition (GLAD) technique for the detection of H2S gas emitted by bacteria. The colorimetric and water wettability features of AgNRs were used to detect live and dead bacteria.
2:Sample Selection and Data Sources:
Four bacteria (E. coli, P. aeruginosa, B. subtilis, and S. aureus) were used as model organisms. E. coli was transfected with pUC19 plasmid vector having an ampicillin-resistant gene for antibiotic resistance studies.
3:List of Experimental Equipment and Materials:
AgNRs arrays on glass substrates, Luria broth (LB) culture medium, ampicillin, Erlenmeyer flasks, autoclave, orbital shaker, fluorescence microscope, Kruss drop shape analyzer, mobile app 'Colorimetric Detector'.
4:Experimental Procedures and Operational Workflow:
Bacterial cultures were grown in LB media with and without ampicillin. AgNRs arrays were exposed to bacterial cultures to detect H2S emission. Changes in color and wettability were measured after 6 and 16 hours.
5:Data Analysis Methods:
The change in color was measured in terms of optical darkness ratio (ODR) using a mobile app. Wettability was measured by contact angle (CA) analysis. Results were verified with fluorescence imaging and standard plate count (SPC) methods.
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