研究目的
Detection of Zn2+ and Cu2+ ions in aqueous solution and live cells using a novel Schiff base chemosensor HMID.
研究成果
The novel Schiff base chemosensor HMID was successfully developed for the selective detection of Zn2+ and Cu2+ ions. HMID can operate as a fluorescence sensor for Zn2+ with a detection limit below the WHO guideline and as a colorimetric sensor for Cu2+. The sensor was also applicable for live cell imaging and as a colorimetric test kit, demonstrating its practical utility.
研究不足
The study mentions that Cu2+, Cr3+, and Co2+ interfered with the interaction between HMID and Zn2+, and Fe2+, Fe3+, and Ni2+ quenched more than half of the fluorescence intensity obtained with Zn2+ alone. The pH effect study showed that HMID with Zn2+ displayed a significant fluorescence enhancement between pH 7.0 and 9.0.
1：Experimental Design and Method Selection:
The study involved the design and synthesis of a Schiff base chemosensor HMID for the detection of Zn2+ and Cu2+ ions. The methodology included fluorescence and UV-vis spectroscopy for ion detection, DFT calculations for understanding the detection mechanisms, and live cell imaging for practical application.
2：Sample Selection and Data Sources:
The chemosensor HMID was synthesized by condensation reaction of 1-aminohydantoin hydrochloride and 3-methoxysalicylaldehyde. Metal ions were detected in aqueous solutions and live cells.
3：List of Experimental Equipment and Materials:
Equipment included a Varian spectrometer for NMR, Perkin Elmer 25 UV-Vis and LS45 fluorescence spectrometers, Thermo MAX instrument for ESI-MS, MICRO CUBE elemental analyzer, and Agilent Cary 670 FTIR spectrometer.
4：Experimental Procedures and Operational Workflow:
The synthesis of HMID, fluorescent and UV-vis titrations with Zn2+ and Cu2+, competition experiments, pH effect studies, live cell imaging, and colorimetric test kit preparation were conducted.
5：Data Analysis Methods:
Data analysis included binding constant calculation using Benesi-Hildebrand equation, detection limit calculation, and DFT calculations for understanding the detection mechanisms.
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