研究目的
Developing a high-resolution, acoustic resolution photoacoustic microscopy (AR-PAM) system in the near infra-red (NIR) window II (NIR-II, e.g., 1064 nm) for deep tissue imaging.
研究成果
The developed 1064-AR-PAM system provides high resolution (130 μm lateral and 57 μm axial) imaging capability and an imaging depth of 11 mm. It has been successfully applied for sentinel lymph node and urinary bladder imaging in rats, demonstrating its potential for deep tissue imaging applications.
研究不足
The blood vessel structures are not as clearly visible as reported by systems using visible or NIR-I wavelength as excitation due to poor light absorption of 1064 nm wavelength. The scanning time is currently ~30 minutes for in vivo imaging of an area of 2 x 2 cm.
1：Experimental Design and Method Selection:
The AR-PAM system was designed with a 1064 nm pulsed laser for illumination, utilizing dark-field light illumination and high numerical aperture ultrasound transducers for detection.
2：Sample Selection and Data Sources:
Carbon microspheres and USAF 1951 resolution test target were used for system characterization. In vivo imaging was performed on sentinel lymph node and urinary bladder of rats.
3：List of Experimental Equipment and Materials:
Nd:YAG laser, ultrasound transducer, optical condenser, carbon microspheres, black ink, etc.
4：Experimental Procedures and Operational Workflow:
The system was characterized for resolution and depth of imaging. In vivo imaging was conducted with contrast agent injection.
5：Data Analysis Methods:
Hilbert transformation was performed on the depth resolved PA signals, followed by post-processing to form maximum amplitude projection (MAP) and depth-encoded MAP (DE-MAP) images.
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