研究目的
Developing a facile test strip for visual detection of dopamine (DA) in biofluids, leveraging dual-emission fluorescent molecularly imprinted polymer nanoparticles (DE-MIPs) for rapid, on-site detection with minimal sample requirement.
研究成果
The developed DA test strip, based on DE-MIPs, offers a novel, efficient, and facile strategy for the rapid, visual, and semiquantitative detection of DA in biofluids. It demonstrates good sensitivity, specificity, and anti-interference ability, with potential applications in clinical diagnostics and point-of-care testing.
研究不足
The study's limitations include the potential for DA oxidation and polymerization under UV light, although the exposure time was minimized to mitigate this effect. Additionally, the test strip's performance in complex biofluids other than serum was not extensively evaluated.
1：Experimental Design and Method Selection:
The study utilized dual-emission fluorescent molecularly imprinted polymer nanoparticles (DE-MIPs) for DA detection, combining two types of quantum dots (QDs) with different color emissions through a molecular imprinting process.
2：Sample Selection and Data Sources:
Human serum samples were used to evaluate the test strip's performance.
3：List of Experimental Equipment and Materials:
Included CdTe QDs, carbon QDs, silica nanoparticles, and various chemicals for polymer synthesis.
4：Experimental Procedures and Operational Workflow:
DE-MIPs were prepared by surface imprinting of DA on silica nanoparticles using CdTe QDs as variable fluorescence signal. The DE-MIPs were then coated on filter paper to create the test strip.
5：Data Analysis Methods:
Fluorescence spectra were analyzed using a fluorescence spectrophotometer, and visual detection was assessed under UV light.
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