研究目的
Investigating the trace of dye molecules in living plants using NaGdF4:Yb3+,Er3+ fluorescent nanoprobes.
研究成果
The NaGdF4:Yb3+,Er3+ nanoparticles effectively traced RhB in bean sprouts, showing a position-dependent absorption effect. The FRET-based method provided a sensitive way to detect residual dyes in plants, with potential applications in food safety and environmental monitoring.
研究不足
The study focuses on RhB detection in bean sprouts, and the applicability to other plants or dyes is not explored. The sensitivity and detection limits are specific to the experimental conditions described.
1：Experimental Design and Method Selection:
The study used NaGdF4:Yb3+,Er3+ nanoparticles as fluorescent probes to trace rhodamine B (RhB) in bean sprouts. The methodology involved the synthesis of nanoparticles, their application in detecting RhB, and the analysis of the fluorescence resonance energy transfer (FRET) effect.
2：Sample Selection and Data Sources:
Bean sprouts were grown in RhB solutions of various concentrations. The roots and stems of the sprouts were analyzed separately.
3：List of Experimental Equipment and Materials:
The synthesis involved chemicals like oleic acid, octadecene, NaOH, NH4F, methanol, polyethylene glycol, rhodamine-B, and RECl3·6H2O. Characterization was done using an H-7650C transmission electron microscope (TEM) and a Hitachi F-2700 spectrophotometer.
4：2O. Characterization was done using an H-7650C transmission electron microscope (TEM) and a Hitachi F-2700 spectrophotometer. Experimental Procedures and Operational Workflow:
4. Experimental Procedures and Operational Workflow: The nanoparticles were synthesized via a solvothermal method. Bean sprouts were grown in RhB solutions, and their cell juice was extracted and mixed with UCNPs for analysis.
5：Data Analysis Methods:
The upconversion spectra and fluorescent images were analyzed to understand the FRET effect and the distribution of RhB in the sprouts.
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