研究目的
To propose a simple and convenient SERS detection of DNA complementarity on large-scale gold gratings for label-free DNA identification.
研究成果
The SPP supported functional platform was proposed as an effective tool for label-free SERS-based DNA recognition. The DNA analysis selectivity was realized through Au grating grafting with single stranded oligonucleotide. The application of PCA analysis allows to clearly distinguish presence of complementary, mispaired or noncomplementary oligonucleotides. DNA detection with the proposed functional SERS platform exhibits excellent results reproducibility and the detection limit of about 10-14 M. The possibility to identify the presence of target oligonucleotide in the oligonucleotides mixture was also demonstrated. The created SERS substrates are favorable by their large scale and the possibility to perform SERS analysis using the portable Raman spectrometer.
研究不足
The utilization of the same algorithm for the detection of larger ss-DNAs molecules can be potentially complicated by the SERS band interference.
1：Experimental Design and Method Selection:
The SERS platform was based on the functional surface plasmon polariton supported ordered structure created by excimer laser patterning and thin Au layer sputtering. The selectivity was achieved through surface grafting with a single DNA chain.
2：Sample Selection and Data Sources:
Oligonucleotides (ODN) were obtained from Biosynthesis (Russia). The immobilization of probe nucleotide on the gold grating surface was performed in a three-step procedure.
3：List of Experimental Equipment and Materials:
Materials included acetic acid, diethyl ether, deionized water, 4-aminobenzoic acid, p-Toluenesulfonic acid monohydrate, high-purity water, and oligonucleotides. Equipment included X-ray photoelectron spectroscopy (XPS), AFM technique with the Icon (Brucker) microscope, scanning electron microscopy (SEM), gel electrophoresis, and a portable ProRaman-L spectrometer.
4：Experimental Procedures and Operational Workflow:
The grafting of probe ODN was performed through EDC/sulfo-NHS activation. Raman scattering was measured on a portable ProRaman-L spectrometer.
5：Data Analysis Methods:
Principal component analysis (PCA) of raw SERS data was used for data evaluation.
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