研究目的
Detection and quantification of mercury ions and biothiols such as Cys, Hcy, GSH in biological and environmental samples contribute to monitoring and preventing disease.
研究成果
A ratiometric fluorescent nanosensor for the detection of Hg2+ and three biothiols was successfully designed and prepared. The nanosensor exhibited high selectivity and sensitivity towards Hg2+ with a detection limit of 97 nM. It also demonstrated the ability to detect biothiols (Cys, Hcy, GSH) with detection limits of 0.133 μM, 0.086 μM, and 0.123 μM, respectively. The nanosensor showed excellent reversibility and potential for practical applications in environmental and biological monitoring.
研究不足
The study is limited to the detection of Hg2+ and specific biothiols (Cys, Hcy, GSH) in aqueous solutions. The sensitivity and selectivity of the nanosensor may vary in complex biological or environmental matrices.
1：Experimental Design and Method Selection:
The study involved the synthesis of a dual-emission nanosensor for Hg2+ detection by coupling CA-AEAPMS on the surface of RBS-doped modified silica microspheres. The methodology included the synthesis of CA-AEAPMS using N-(β-aminoethyl)-γ-aminopropyl methyldimethoxysilane (AEAPMS) and citric acid as the main raw materials.
2：Sample Selection and Data Sources:
The samples used were silica microspheres doped with RBS and coated with CA-AEAPMS. Data was collected using fluorescence spectrometry.
3：List of Experimental Equipment and Materials:
Equipment included a Fluoromax-4 fluorescence spectrometer, low-temperature reaction bath DHJF-4005, ultrasound machine KQ3200DB, and TG16G high-speed centrifuge. Materials included N-(β-aminoethyl)-γ-aminopropyl methyldimethoxysilane (AEAPMS), anhydrous citric acid (CA), aminopropyltriethoxysilane (APTES), and others.
4：Experimental Procedures and Operational Workflow:
The procedure involved the synthesis of RBS-doped silica microspheres, coating them with CA-AEAPMS, and testing their fluorescence response to Hg2+ and biothiols.
5：Data Analysis Methods:
The fluorescence quenching was analyzed using the Stern–Volmer equation to determine the detection limits for Hg2+ and biothiols.
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